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Impairment of the b...
Impairment of the biomechanical compliance of P pili : a novel means of inhibiting uropathogenic bacterial infections?
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- Klinth, Jeanna E (författare)
- Umeå universitet,Institutionen för fysik,Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Molekylär Infektionsmedicin, Sverige (MIMS),Centrum för medicinsk teknik och fysik (CMTF)
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Pinkner, Jerome S (författare)
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Hultgren, Scott J (författare)
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- Almqvist, Fredrik (författare)
- Umeå universitet,Kemiska institutionen,Umeå Centre for Microbial Research (UCMR)
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- Uhlin, Bernt Eric (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Umeå Centre for Microbial Research (UCMR),Molekylär Infektionsmedicin, Sverige (MIMS),Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Axner, Ove (författare)
- Umeå universitet,Institutionen för fysik,Umeå Centre for Microbial Research (UCMR),Centrum för medicinsk teknik och fysik (CMTF)
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(creator_code:org_t)
- 2012-01-12
- 2012
- Engelska.
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Ingår i: European Biophysics Journal. - Berlin : Springer. - 0175-7571 .- 1432-1017. ; 41:3, s. 285-295
- Relaterad länk:
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https://umu.diva-por... (primary) (Raw object)
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https://link.springe...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Gram-negative bacteria often initiate their colonization by use of extended attachment organelles, so called pili. When exposed to force, the rod of helix-like pili has been found to be highly extendable, mainly attributed to uncoiling and recoiling of its quaternary structure. This provides the bacteria with the ability to redistribute an external force among a multitude of pili, which enables them to withstand strong rinsing flows, which, in turn, facilitates adherence and colonization processes critical to virulence. Thus, pili fibers are possible targets for novel antibacterial agents. By use of a substance that compromises compliance of the pili, the ability of bacteria to redistribute external forces can be impaired, so they will no longer be able to resist strong urine flow and thus be removed from the host. It is possible such a substance can serve as an alternative to existing antibiotics in the future or be a part of a multi-drug. In this work we investigated whether it is possible to achieve this by targeting the recoiling process. The test substance was purified PapD. The effect of PapD on the compliance of P pili was assessed at the single organelle level by use of force-measuring optical tweezers. We showed that the recoiling process, and thus the biomechanical compliance, in particular the recoiling process, can be impaired by the presence of PapD. This leads to a new concept in the search for novel drug candidates combating uropathogenic bacterial infections-"coilicides", targeting the subunits of which the pilus rod is composed.
Ämnesord
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Bacterial adhesion
- Force-measuring optical tweezers
- Antimicrobial
- Single organelle
- Pili recoiling
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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