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Evidence of the TNF...
Evidence of the TNF-α system in the human Achilles tendon : expression of TNF-α and TNF receptor at both protein and mRNA levels in the tenocytes
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- Gaida, James Edmund (författare)
- Umeå universitet,Anatomi,Idrottsmedicin
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- Bagge, Johan (författare)
- Umeå universitet,Anatomi
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Purdam, Craig (författare)
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Cook, Jill (författare)
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- Alfredson, Håkan (författare)
- Umeå universitet,Idrottsmedicin
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- Forsgren, Sture (författare)
- Umeå universitet,Anatomi
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(creator_code:org_t)
- 2012-05-08
- 2012
- Engelska.
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Ingår i: Cells Tissues Organs. - : S. Karger. - 1422-6405 .- 1422-6421. ; 196:4, s. 339-352
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Understanding adaption to load is essential for prevention and treatment of tendinopathy/tendinosis. Cytokine release in response to load is one mechanism involved in mechanotransduction. The cytokine tumor necrosis factor alpha (TNF-α) is implicated in tendinosis and can induce apoptotic effects via tumor necrosis factor receptor 1 (TNFR1). The complete absence of information concerning the TNF-α system in Achilles tendon is a limitation as mid-portion Achilles tendinosis is very frequent. Purpose: To examine expression patterns of TNF-α and its two receptors (TNFR1 and TNFR2) in human Achilles tendinosis and control tissue and to biochemically confirm the presence of TNF-α in tendinosis tissue. Methods: TNF-α and TNFR1 mRNA were detected via in situ hybridization. TNF-α, TNFR1, and TNFR2 were demonstrated immunohistochemically. Apoptosis markers were utilized. ELISA was used to detect TNF-α. Results: TNF-α and TNFR1 mRNA was detected in tenocytes of both tendinosis and control tendons. Tenocytes from both groups displayed specific immunoreactions for TNF-α, TNFR1, and TNFR2. The widened/rounded tenocytes of tendinosis samples exhibited the most intense immunoreactions. Apoptosis was detected in only a subpopulation of the tenocytes in tendinosis tissue. TNF-α was measurable in tendinosis tissue. Inflammatory cells were not seen. Conclusion: This is the first evidence of the existence of the TNF-α system in the human Achilles tendon. Findings are confirmed at mRNA and protein levels as well as biochemically. The TNF-α system was in principle confined to the tenocytes. The connection between tenocyte morphology and the expression pattern of TNF-α, TNFR1, and TNFR2 suggests that the TNF-α system may be involved in tenocyte activation in Achilles tendinosis.
Nyckelord
- Achilles tendon
- Tendinopathy
- Cytokine
- TNF-α
- TNFR1
- TNFR2
- Apoptosis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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