An early role for WNT signaling in specifying neural patterns of Cdx and Hox gene expression and motor neuron subtype identity

Nordström, Ulrika,1971-Umeå universitet,Umeå centrum för molekylär medicin (UCMM) (author)

The link between extrinsic signaling, progenitor cell specification and neuronal subtype identity is central to the developmental organization of the vertebrate central nervous system. In the hindbrain and spinal cord, distinctions in the rostrocaudal identity of progenitor cells are associated with the generation of different motor neuron subtypes. Two fundamental classes of motor neurons, those with dorsal (dMN) and ventral (vMN) exit points, are generated over largely non-overlapping rostrocaudal domains of the caudal neural tube. Cdx and Hox genes are important determinants of the rostrocaudal identity of neural progenitor cells, but the link between early patterning signals, neural Cdx and Hox gene expression, and the generation of dMN and vMN subtypes, is unclear. Using an in vitro assay of neural differentiation, we provide evidence that an early Wnt-based program is required to interact with a later retinoic acid- and fibroblast growth factor–mediated mechanism to generate a pattern of Cdx and Hox profiles characteristic of hindbrain and spinal cord progenitor cells that prefigure the generation of vMNs and dMNs.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Animals
Avian Proteins/genetics/metabolism
Central Nervous System/*embryology
Chick Embryo
Fibroblast Growth Factors/metabolism
Gene Expression Regulation; Developmental
Genes; Homeobox
Homeodomain Proteins/genetics/*metabolism
Motor Neurons/*cytology/metabolism
Rhombencephalon/cytology/embryology/metabolism
Signal Transduction
Spinal Cord/cytology/embryology/metabolism
Stem Cells/cytology/metabolism
T-Box Domain Proteins/genetics/metabolism
Tretinoin/metabolism
Wnt Proteins/genetics/*metabolism

Maier, Esther,1974-Umeå universitet,Umeå centrum för molekylär medicin (UCMM) (author)
Jessell, Thomas M (author)
Edlund, ThomasUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)thed0001 (author)
Umeå universitetUmeå centrum för molekylär medicin (UCMM) (creator_code:org_t)

By the author/editor: Nordström, Ulrik ...; Maier, Esther, 1 ...; Jessell, Thomas ...; Edlund, Thomas

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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