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Genetic dissection ...
Genetic dissection of the circuit for hand dexterity in primates
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- Kinoshita, Masaharu (författare)
- National Institute for Physiological Sciences, Myodaiji, Okazaki
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- Matsui, Ryosuke (författare)
- Kyoto University
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- Kato, Shigeki (författare)
- Fukushima Medical University School of Medicine, Fukushima
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- Hasegawa, Taku (författare)
- Kyoto University
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- Kasahara, Hironori (författare)
- Kyoto University
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- Isa, Kaoru (författare)
- National Institute for Physiological Sciences, Myodaiji, Okazaki
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- Watakabe, Akiya (författare)
- National Institute for Basic Biology, Okazaki, he Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa
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- Yamamori, Tetsuo (författare)
- National Institute for Basic Biology, Okazaki, he Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa
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- Nishimura, Yukio (författare)
- National Institute for Physiological Sciences, Myodaiji, Okazaki
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- Alstermark, Bror (författare)
- Umeå universitet,Fysiologi
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- Watanabe, Dai (författare)
- Kyoto University
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- Kobayashi, Kazuto (författare)
- Fukushima Medical University School of Medicine, Fukushima
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- Isa, Tadashi (författare)
- National Institute for Physiological Sciences, Myodaiji, Okazaki , he Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa
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(creator_code:org_t)
- 2012-06-17
- 2012
- Engelska.
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 487:7406, s. 235-U1510
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- It is generally accepted that the direct connection from the motor cortex to spinal motor neurons is responsible for dexterous hand movements in primates(1-3). However, the role of the 'phylogenetically older' indirect pathways from the motor cortex to motor neurons, mediated by spinal interneurons, remains elusive. Here we used a novel double-infection technique to interrupt the transmission through the propriospinal neurons (PNs)(4-6), which act as a relay of the indirect pathway in macaque monkeys (Macaca fuscata and Macaca mulatta). The PNs were double infected by injection of a highly efficient retrograde gene-transfer vector into their target area and subsequent injection of adeno-associated viral vector at the location of cell somata. This method enabled reversible expression of green fluorescent protein (GFP)-tagged tetanus neurotoxin, thereby permitting the selective and temporal blockade of the motor cortex-PN-motor neuron pathway. This treatment impaired reach and grasp movements, revealing a critical role for the PN-mediated pathway in the control of hand dexterity. Anti-GFP immunohistochemistry visualized the cell bodies and axonal trajectories of the blocked PNs, which confirmed their anatomical connection to motor neurons. This pathway-selective and reversible technique for blocking neural transmission does not depend on cell-specific promoters or transgenic techniques, and is a new and powerful tool for functional dissection in system-level neuroscience studies.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
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Kinoshita, Masah ...
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Matsui, Ryosuke
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Kato, Shigeki
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Hasegawa, Taku
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Kasahara, Hirono ...
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Isa, Kaoru
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visa fler...
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Watakabe, Akiya
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Yamamori, Tetsuo
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Nishimura, Yukio
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Alstermark, Bror
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Watanabe, Dai
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Kobayashi, Kazut ...
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Isa, Tadashi
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