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Genetic dissection ...
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Kinoshita, MasaharuNational Institute for Physiological Sciences, Myodaiji, Okazaki
(författare)
Genetic dissection of the circuit for hand dexterity in primates
- Artikel/kapitelEngelska2012
Förlag, utgivningsår, omfång ...
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2012-06-17
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Springer Science and Business Media LLC,2012
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:umu-61229
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-61229URI
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https://doi.org/10.1038/nature11206DOI
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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It is generally accepted that the direct connection from the motor cortex to spinal motor neurons is responsible for dexterous hand movements in primates(1-3). However, the role of the 'phylogenetically older' indirect pathways from the motor cortex to motor neurons, mediated by spinal interneurons, remains elusive. Here we used a novel double-infection technique to interrupt the transmission through the propriospinal neurons (PNs)(4-6), which act as a relay of the indirect pathway in macaque monkeys (Macaca fuscata and Macaca mulatta). The PNs were double infected by injection of a highly efficient retrograde gene-transfer vector into their target area and subsequent injection of adeno-associated viral vector at the location of cell somata. This method enabled reversible expression of green fluorescent protein (GFP)-tagged tetanus neurotoxin, thereby permitting the selective and temporal blockade of the motor cortex-PN-motor neuron pathway. This treatment impaired reach and grasp movements, revealing a critical role for the PN-mediated pathway in the control of hand dexterity. Anti-GFP immunohistochemistry visualized the cell bodies and axonal trajectories of the blocked PNs, which confirmed their anatomical connection to motor neurons. This pathway-selective and reversible technique for blocking neural transmission does not depend on cell-specific promoters or transgenic techniques, and is a new and powerful tool for functional dissection in system-level neuroscience studies.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Matsui, RyosukeKyoto University
(författare)
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Kato, ShigekiFukushima Medical University School of Medicine, Fukushima
(författare)
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Hasegawa, TakuKyoto University
(författare)
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Kasahara, HironoriKyoto University
(författare)
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Isa, KaoruNational Institute for Physiological Sciences, Myodaiji, Okazaki
(författare)
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Watakabe, AkiyaNational Institute for Basic Biology, Okazaki, he Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa
(författare)
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Yamamori, TetsuoNational Institute for Basic Biology, Okazaki, he Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa
(författare)
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Nishimura, YukioNational Institute for Physiological Sciences, Myodaiji, Okazaki
(författare)
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Alstermark, BrorUmeå universitet,Fysiologi(Swepub:umu)bral0001
(författare)
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Watanabe, DaiKyoto University
(författare)
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Kobayashi, KazutoFukushima Medical University School of Medicine, Fukushima
(författare)
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Isa, TadashiNational Institute for Physiological Sciences, Myodaiji, Okazaki , he Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa
(författare)
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National Institute for Physiological Sciences, Myodaiji, OkazakiKyoto University
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Nature: Springer Science and Business Media LLC487:7406, s. 235-U15100028-08361476-4687
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Nature
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Till lärosätets databas
- Av författaren/redakt...
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Kinoshita, Masah ...
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Matsui, Ryosuke
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Kato, Shigeki
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Hasegawa, Taku
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Kasahara, Hirono ...
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Isa, Kaoru
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visa fler...
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Watakabe, Akiya
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Yamamori, Tetsuo
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Nishimura, Yukio
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Alstermark, Bror
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Watanabe, Dai
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Kobayashi, Kazut ...
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Isa, Tadashi
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
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Umeå universitet