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Sökning: WFRF:(Kobayashi Shigeki) > (2012) > Genetic dissection ...

  • Kinoshita, MasaharuNational Institute for Physiological Sciences, Myodaiji, Okazaki (författare)

Genetic dissection of the circuit for hand dexterity in primates

  • Artikel/kapitelEngelska2012

Förlag, utgivningsår, omfång ...

  • 2012-06-17
  • Springer Science and Business Media LLC,2012
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:umu-61229
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-61229URI
  • https://doi.org/10.1038/nature11206DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • It is generally accepted that the direct connection from the motor cortex to spinal motor neurons is responsible for dexterous hand movements in primates(1-3). However, the role of the 'phylogenetically older' indirect pathways from the motor cortex to motor neurons, mediated by spinal interneurons, remains elusive. Here we used a novel double-infection technique to interrupt the transmission through the propriospinal neurons (PNs)(4-6), which act as a relay of the indirect pathway in macaque monkeys (Macaca fuscata and Macaca mulatta). The PNs were double infected by injection of a highly efficient retrograde gene-transfer vector into their target area and subsequent injection of adeno-associated viral vector at the location of cell somata. This method enabled reversible expression of green fluorescent protein (GFP)-tagged tetanus neurotoxin, thereby permitting the selective and temporal blockade of the motor cortex-PN-motor neuron pathway. This treatment impaired reach and grasp movements, revealing a critical role for the PN-mediated pathway in the control of hand dexterity. Anti-GFP immunohistochemistry visualized the cell bodies and axonal trajectories of the blocked PNs, which confirmed their anatomical connection to motor neurons. This pathway-selective and reversible technique for blocking neural transmission does not depend on cell-specific promoters or transgenic techniques, and is a new and powerful tool for functional dissection in system-level neuroscience studies.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Matsui, RyosukeKyoto University (författare)
  • Kato, ShigekiFukushima Medical University School of Medicine, Fukushima (författare)
  • Hasegawa, TakuKyoto University (författare)
  • Kasahara, HironoriKyoto University (författare)
  • Isa, KaoruNational Institute for Physiological Sciences, Myodaiji, Okazaki (författare)
  • Watakabe, AkiyaNational Institute for Basic Biology, Okazaki, he Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa (författare)
  • Yamamori, TetsuoNational Institute for Basic Biology, Okazaki, he Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa (författare)
  • Nishimura, YukioNational Institute for Physiological Sciences, Myodaiji, Okazaki (författare)
  • Alstermark, BrorUmeå universitet,Fysiologi(Swepub:umu)bral0001 (författare)
  • Watanabe, DaiKyoto University (författare)
  • Kobayashi, KazutoFukushima Medical University School of Medicine, Fukushima (författare)
  • Isa, TadashiNational Institute for Physiological Sciences, Myodaiji, Okazaki , he Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa (författare)
  • National Institute for Physiological Sciences, Myodaiji, OkazakiKyoto University (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Nature: Springer Science and Business Media LLC487:7406, s. 235-U15100028-08361476-4687

Internetlänk

Hitta via bibliotek

  • Nature (Sök värdpublikationen i LIBRIS)

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