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Functional corpora ...
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Wahlberg, PatrikUmeå universitet,Institutionen för medicinsk kemi och biofysik
(author)
Functional corpora lutea are formed in matrix metalloproteinase inhibitor-treated plasminogen-deficient mice.
- Article/chapterEnglish2007
Publisher, publication year, extent ...
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-6326
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-6326URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The extended Förster theory (EFT) is for the first time applied to the quantitative determination of the intramolecular distances in proteins. It is shown how the EFT (J. Chem. Phys., 1996, 105, 10896) can be adapted to the analyses of fluorescence depolarisation experiments based on the time-correlated single photon counting technique (TCSPC). The protein system studied was the latent form of plasminogen activator inhibitor type I (PAI-1), which was mutated and labelled by the thiol reactive BODIPY® derivative {N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-yl)methyl iodoacetamide}. The energy migration occurs within pairs of photophysically identical donor groups that undergo reorientational motions on the timescales of energy migration and fluorescence relaxation. Unlike all models currently used for analysing fluorescence TCSPC data, the EFT explicitly accounts for the time-dependent reorientations that influence the rate of electronic energy transfer/migration in a complex manner. The complexity is related to the 2 problem, which has been discussed for years. The EFT brings the analyses of DDEM data to the same level of molecular description as in ESR and NMR spectroscopy, i.e. it yields microscopic information about the reorientation correlation times, the order parameters, as well as inter-chromophoric distances.
Subject headings and genre
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Animals
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Corpus Luteum/blood supply/*drug effects/*physiology
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Dipeptides/pharmacology
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Female
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Gonadotropins/pharmacology
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Male
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Matrix Metalloproteinases/*antagonists & inhibitors
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Mice
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Mice; Inbred C57BL
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Mice; Knockout
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Plasminogen/*genetics
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Progesterone/blood
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Pseudopregnancy/blood
Added entries (persons, corporate bodies, meetings, titles ...)
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Bodén, Ida
(author)
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Paulsson, Josefin
(author)
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Lund, Leif R
(author)
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Liu, KuiUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)kuli0001
(author)
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Ny, TorUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)tony0001
(author)
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Umeå universitetInstitutionen för medicinsk kemi och biofysik
(creator_code:org_t)
Related titles
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In:Endocrinology148:3, s. 1226-340013-7227
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