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Idling by DNA polym...
Idling by DNA polymerase delta maintains a ligatable nick during lagging-strand DNA replication.
- Article/chapterEnglish2004
Publisher, publication year, extent ...
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2004-11-01
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Cold Spring Harbor Laboratory,2004
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-6352
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-6352URI
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https://doi.org/10.1101/gad.1252304DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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During each yeast cell cycle, ∼100,000 nicks are generated during lagging-strand DNA replication. Efficient nick processing during Okazaki fragment maturation requires the coordinated action of DNA polymerase δ (Pol δ) and the FLAP endonuclease FEN1. Misregulation of this process leads to the accumulation of double-stranded breaks and cell lethality. Our studies highlight a remarkably efficient mechanism for Okazaki fragment maturation in which Pol δ by default displaces 2–3 nt of any downstream RNA or DNA it encounters. In the presence of FEN1, efficient nick translation ensues, whereby a mixture of mono- and small oligonucleotides are released. If FEN1 is absent or not optimally functional, the ability of Pol δ to back up via its 3′–5′-exonuclease activity, a process called idling, maintains the polymerase at a position that is ideal either for ligation (in case of a DNA–DNA nick) or for subsequent engagement by FEN1 (in case of a DNA–RNA nick). Consistent with the hypothesis that DNA polymerase ϵ is the leading-strand enzyme, we observed no idling by this enzyme and no cooperation with FEN1 for creating a ligatable nick.
Subject headings and genre
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DNA/*metabolism
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DNA Helicases/metabolism
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DNA Polymerase III/*metabolism
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DNA Primers
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DNA Replication
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Exonucleases/metabolism
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Flap Endonucleases/*metabolism
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Oligonucleotides/metabolism
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Saccharomyces cerevisiae/*enzymology/*genetics
Added entries (persons, corporate bodies, meetings, titles ...)
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Stith, Carrie M
(author)
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Sabouri, NasimUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)namsai01
(author)
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Johansson, ErikUmeå universitet,Institutionen för medicinsk kemi och biofysik(Swepub:umu)erjo0002
(author)
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Burgers, Peter M
(author)
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Umeå universitetInstitutionen för medicinsk kemi och biofysik
(creator_code:org_t)
Related titles
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In:Genes & Development: Cold Spring Harbor Laboratory18:22, s. 2764-27730890-93691549-5477
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