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  • Eriksson, Anna U.Umeå universitet,Umeå centrum för molekylär medicin (UCMM) (author)

Near infrared optical projection tomography for assessments of beta-cell mass distribution in diabetes research

  • Article/chapterEnglish2013

Publisher, publication year, extent ...

  • 2013-01-12
  • MyJove Corporation,2013
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:umu-64029
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-64029URI
  • https://doi.org/10.3791/50238DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • By adapting OPT to include the capability of imaging in the near infrared (NIR) spectrum, we here illustrate the possibility to image larger bodies of pancreatic tissue, such as the rat pancreas, and to increase the number of channels (cell types) that may be studied in a single specimen. We further describe the implementation of a number of computational tools that provide: 1/ accurate positioning of a specimen's (in our case the pancreas) centre of mass (COM) at the axis of rotation (AR)2; 2/ improved algorithms for post-alignment tuning which prevents geometric distortions during the tomographic reconstruction2 and 3/ a protocol for intensity equalization to increase signal to noise ratios in OPT-based BCM determinations3. In addition, we describe a sample holder that minimizes the risk for unintentional movements of the specimen during image acquisition. Together, these protocols enable assessments of BCM distribution and other features, to be performed throughout the volume of intact pancreata or other organs (e.g. in studies of islet transplantation), with a resolution down to the level of individual islets of Langerhans.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Svensson, ChristofferUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)chsv0004 (author)
  • Hörnblad, AndreasUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)anshod02 (author)
  • Cheddad, AbbasUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)abch0002 (author)
  • Kostromina, ElenaUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)elko0010 (author)
  • Eriksson, MariaUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)maer0057 (author)
  • Norlin, NilsUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)nino0001 (author)
  • Pileggi, AntonelloCell Transplants Center, Diabetes Research Institute, University of Miami (author)
  • Sharpe, JamesEMBL-CRG Systems Biology Program, Centre for Genomic Regulation, Catalan Institute of Research and Advance Studies (author)
  • Georgsson, Fredrik,1971-Umeå universitet,Institutionen för datavetenskap(Swepub:umu)frge0001 (author)
  • Alanentalo, TomasUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)tosalo98 (author)
  • Ahlgren, UlfUmeå universitet,Umeå centrum för molekylär medicin (UCMM)(Swepub:umu)ulah0001 (author)
  • Umeå universitetUmeå centrum för molekylär medicin (UCMM) (creator_code:org_t)

Related titles

  • In:Journal of Visualized Experiments: MyJove Corporation711940-087X

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