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Near infrared optic...
Near infrared optical projection tomography for assessments of beta-cell mass distribution in diabetes research
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- Eriksson, Anna U. (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Svensson, Christoffer (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Hörnblad, Andreas (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Cheddad, Abbas (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Kostromina, Elena (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Eriksson, Maria (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Norlin, Nils (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Pileggi, Antonello (author)
- Cell Transplants Center, Diabetes Research Institute, University of Miami
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- Sharpe, James (author)
- EMBL-CRG Systems Biology Program, Centre for Genomic Regulation, Catalan Institute of Research and Advance Studies
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- Georgsson, Fredrik, 1971- (author)
- Umeå universitet,Institutionen för datavetenskap
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- Alanentalo, Tomas (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Ahlgren, Ulf (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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(creator_code:org_t)
- 2013-01-12
- 2013
- English.
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In: Journal of Visualized Experiments. - : MyJove Corporation. - 1940-087X. ; 71
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https://doi.org/10.3...
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https://umu.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- By adapting OPT to include the capability of imaging in the near infrared (NIR) spectrum, we here illustrate the possibility to image larger bodies of pancreatic tissue, such as the rat pancreas, and to increase the number of channels (cell types) that may be studied in a single specimen. We further describe the implementation of a number of computational tools that provide: 1/ accurate positioning of a specimen's (in our case the pancreas) centre of mass (COM) at the axis of rotation (AR)2; 2/ improved algorithms for post-alignment tuning which prevents geometric distortions during the tomographic reconstruction2 and 3/ a protocol for intensity equalization to increase signal to noise ratios in OPT-based BCM determinations3. In addition, we describe a sample holder that minimizes the risk for unintentional movements of the specimen during image acquisition. Together, these protocols enable assessments of BCM distribution and other features, to be performed throughout the volume of intact pancreata or other organs (e.g. in studies of islet transplantation), with a resolution down to the level of individual islets of Langerhans.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Eriksson, Anna U ...
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Svensson, Christ ...
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Hörnblad, Andrea ...
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Cheddad, Abbas
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Kostromina, Elen ...
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Eriksson, Maria
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Norlin, Nils
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Pileggi, Antonel ...
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Sharpe, James
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Georgsson, Fredr ...
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Alanentalo, Toma ...
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Ahlgren, Ulf
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomedical Labor ...
- Articles in the publication
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Journal of Visua ...
- By the university
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Umeå University