Effects of fucosylated milk of goat and mouse on Helicobacter pylori binding to Lewis b antigen

• Aim:To evaluate the effects of animal milk containing fucosylated antigens on Helicobacter pylori (H pylon) binding to Lewis b antigen. Methods:A mammary gland expression vector containing human α1-3/4-fucosyltransferase cDNA sequences was constructed. Transient expression of human(α1-3/4-fucosyltransferase cDNA in goat mammary cell and establishment of transgenic mice were performed. The adhesion inhibitory properties of milk samples were analyzed by using Hpylori. Results: Goat milk samples were found to inhibit bacterial binding to Lewis b antigen. The highest inhibition was observed 42 h after injection of the plasmid. The binding activity of Hpylori to Lewis b antigen reduced mostly, by 83%, however milk samples from transgenic mice did not inhibit Hpylori binding to Lewis b antigen. Conclusion: The use of “humanized“ animal milk produced by the transgenic introduction of fucosylated antigen can perhaps provide an alternative therapy and preventive measure for Hpylori infection.

Nyckelord

Animals

Bacterial Adhesion

Blood Group Antigens/*metabolism

Female

Food; Genetically Modified

Fucosyltransferases/*genetics/metabolism

Gene Expression

Goats

Helicobacter pylori/*metabolism/physiology

Humans

Lewis Blood-Group System/*immunology

Mammary Glands; Animal/enzymology

Mice

Mice; Transgenic

Milk/enzymology/*physiology

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)