Search: (L773:0014 2980 OR L773:1521 4141) srt2:(1990-1999) >
Altered VH6-D-JH re...
Altered VH6-D-JH repertoire in human insulin-dependent diabetes mellitus and autoimmune idiopathic thrombocytopenic purpura
-
- Söderström, Ingegerd (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Cell and Molecular Biology University of Umeå, Umeå, Sweden
-
- van Dijk-Härd, Iris (author)
- Division for Clinical Immunology, Karolinska Institute at Huddinge University Hospital, Huddinge, Sweden
-
- Feld, Sari (author)
- Division for Clinical Immunology, Karolinska Institute at Huddinge University Hospital, Huddinge, Sweden
-
show more...
-
- Hillörn, Valter (author)
- Umeå universitet,Medicin
-
- Holmberg, Dan (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Cell and Molecular Biology University of Umeå, Umeå, Sweden
-
- Lundkvist, Inger (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- Wiley-VCH Verlagsgesellschaft, 1999
- 1999
- English.
-
In: European Journal of Immunology. - : Wiley-VCH Verlagsgesellschaft. - 0014-2980 .- 1521-4141. ; 29:9, s. 2853-2862
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- We have characterized the peripheral B cell repertoire in T cell-mediated insulin-dependent diabetes mellitus (IDMM) and in B cell-mediated autoimmune idiopathic thrombocytopenic purpura (AITP). The VH6-containing repertoire in adult patients with IDDM or AITP and healthy control subjects was investigated by PCR amplification using VH6- and JH-specific primers. Nucleotide sequence analysis of VH6-D-JH rearrangements showed an abnormally high frequency of somatic mutations in non-functional rearrangements from diabetic (3. 58 %) as well as AITP patients (3.18 %), compared to controls (0.4 % and 1.43 %, respectively; p < 0.05). In contrast, the mutation frequency among functional rearrangements was 2.4 - 3 times lower in patients compared to controls ( p < 0.05). Detailed analysis of the VH6 genes carrying mutations showed that the underlying mechanism for this observation is probably different for the two diseases. Analysis of D- and JH gene usage revealed additional deviations from the normal pattern. Taken together, these results suggest defects in the mechanisms controlling selection of the B cell repertoire in patients with IDDM or AITP.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database