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High expression of V gamma 8 is a shared feature of human gamma delta T cells in the epithelium of the gut and in the inflamed synovial tissue.
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Söderström, K (författare)
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Bucht, A (författare)
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Halapi, E (författare)
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Lundqvist, C (författare)
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Grönberg, A (författare)
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Nilsson, E (författare)
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Orsini, D L (författare)
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van de Wal, Y (författare)
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Koning, F (författare)
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- Hammarström, Marie-Louise (författare)
- Umeå universitet,Immunologi/immunkemi
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(creator_code:org_t)
- 1994
- 1994
- Engelska.
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Ingår i: Journal of Immunology. - 0022-1767 .- 1550-6606. ; 152:12, s. 6017-27
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- We have analyzed the V-gene usage in gamma delta T cells of the human gut and joint by using a new mAb (B18) specific for V gamma 8 of human TCR-gamma delta+ T cells. The B18+ population constituted a minor subset of the gamma delta T cells in peripheral blood (PB) of healthy persons (6 +/- 5%) and only 1 of 35 gamma delta T cell clones analyzed was positive. In contrast, the B18+ subset was a dominant gamma delta T cell population among intraepithelial lymphocytes (IEL) derived from the human intestine (74 +/- 29, p < 0.002), and two of three IEL clones from patients with coeliac disease were B18+. Interestingly, a higher proportion of B18+ gamma delta T cells was found in the synovial fluid of patients with rheumatoid arthritis (RA) (21 +/- 18%, 0.02 < p < 0.05) compared with normal PB. Furthermore, the B18+ subset was more frequent among IL-2-expanded gamma delta T cells (42 +/- 20%) derived from synovial tissue than among IL-2-expanded cells derived from synovial fluid (p < 0.002) and PB from RA patients (p < 0.02) as well as normal PB (p < 0.002). The V-gene usage of 13 gamma delta T cell clones from the synovial fluid of arthritic patients was analyzed. All B18+ clones (n = 7) expressed mRNA for V gamma 8 together with mRNA for V delta 1 (n = 5) or mRNA for V delta 3 (n = 2). None of the B18- clones expressed V gamma 8 (n = 6). We conclude that the gamma delta T cell that expresses V gamma 8, together with mainly V delta 1, is a major gamma delta T cell subset among the IEL of the gut and a highly frequent subset in the synovial tissue of patients with RA. This subset may correspond to the mouse V gamma 7+ IEL, which has a high degree of amino acid sequence homology with the human V gamma 8 protein.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
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- ref (ämneskategori)
- art (ämneskategori)
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Söderström, K
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Bucht, A
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Halapi, E
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Lundqvist, C
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Grönberg, A
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Nilsson, E
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visa fler...
-
Orsini, D L
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van de Wal, Y
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Koning, F
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Hammarström, Mar ...
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visa färre...
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Umeå universitet