Two-photon excitation and time-resolved fluorescence: I. The proper response function for analysing single-photon counting experiments

An accurate instrumental response function is needed to deconvolute fluorescence data obtained by time-correlated single-photon counting (TCSPC) upon multi-photon excitation. Hitherto the response function was obtained by measuring Rayleigh scattering (RS) from colloidal solutions, as is also used in one-photon excited fluorescence. We show that hyper Rayleigh scattering (HRS) provides a better choice for deconvolution of fluorescence decays, as obtained by TCSPC and two-photon excitation (TPE). The one- and two-photon response functions were monitored as RS and HRS from colloidal gold particles at 800 and 400 nm, respectively.

Hjelm, Johan (författare)
Mukhtar, Emad (författare)
Bergström, FredrikUmeå universitet,Kemiska institutionen (författare)
Johansson, Lennart B-ÅUmeå universitet,Kemiska institutionen(Swepub:umu)lejo0007 (författare)
Umeå universitetKemiska institutionen (creator_code:org_t)

Av författaren/redakt...: Habenicht, Anja; Hjelm, Johan; Mukhtar, Emad; Bergström, Fredr ...; Johansson, Lenna ...

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