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Cisplatin-resistanc...
Cisplatin-resistance and cell death in malignant pleural mesothelioma cells
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- Janson, Veronica, 1974- (författare)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Grankvist, Kjell, Professor (preses)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Henriksson, Roger, Professor (preses)
- Umeå universitet,Institutionen för strålningsvetenskaper
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- Behnam-Motlagh, Parviz, PhD (preses)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Johansson, Anders, OD (preses)
- Umeå universitet,Institutionen för odontologi
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- Zhivotovsky, Boris, Professor (opponent)
- Institutet för Miljömedicin, Toxikologi, Karolinska Institutet, Stockholm
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(creator_code:org_t)
- ISBN 9789172645608
- Umeå : Medicinsk biovetenskap, 2008
- Engelska 104 s.
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Serie: Umeå University medical dissertations, 0346-6612 ; 1192
- Relaterad länk:
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Malignant pleural mesothelioma (MPM) is an aggressive, treatment-resistant tumour. Cisplatin (cis-diamminedichloroplatinum (II)) is the best single-agent chemotherapy for MPM, but platinum-based combination therapies give the best overall response rates. However, cisplatin use is limited by resistance and severe side effects. This thesis has increased the knowledge concerning cisplatin-induced cell death in MPM by describing a novel potential therapeutic target, and three novel phenotypes of cisplatin-resistance in a human MPM cell line (P31) and its cisplatin-resistant sub-line (P31res1.2). The novel potential therapeutic target, and one of the novel phenotypes, was cisplatin-resistant pro-apoptotic BH3-only proteins. In the P31 cells, cisplatin transiently increased pro-apoptotic BH3-only proteins during 6 h of exposure. This response was almost completely abrogated in the P31res1.2 cells. De-regulated caspase activity and activation was the second novel phenotype identified. The P31res1.2 cells had earlier, possibly mitochondria-independent, caspase-3 activation, increased basal caspase-3 activity and increased basal cleavage of caspase-8 and -9. Despite these differences, 6-h equitoxic cisplatin exposures rendered 50-60% of the cells apoptotic in both cell lines. The third novel phenotype was abrogated Na+K+2Cl--cotransporter (NKCC1) activity. Although NKCC1 activity was dispensable for cisplatin-induced apoptosis, balanced potassium transport activity was essential for P31 cell survival. Finally, the survival signalling protein Protein Kinase B (PKB or Akt) isoforms α and γ were constitutively activated in a PI3K-independent manner in P31 cells. In the P31res1.2 cells, PKBα and γ activities were increased, and there was PI3K-dependent activation of PKBβ. However, this increase in PKB isoform activity was not strongly associated to the cisplatin-resistance of the P31res1.2 cells.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Annan klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Other Clinical Medicine (hsv//eng)
Nyckelord
- apoptosis
- BH3-only proteins
- caspase
- cell morphology
- potassium transport
- protein kinase B (PKB/Akt)
- signalling pathways
- time
- Clinical chemistry
- Klinisk kemi
Publikations- och innehållstyp
- vet (ämneskategori)
- dok (ämneskategori)
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