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Variants within MEC...
Variants within MECP2, a key transcription regulator, are associated with increased susceptibility to lupus and differential gene expression in patients with systemic lupus erythematosus
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Webb, Ryan (author)
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Wren, Jonathan D. (author)
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Jeffries, Matlock (author)
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Kelly, Jennifer A. (author)
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Kaufman, Kenneth M. (author)
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Tang, Yuhong (author)
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Frank, Mark Barton (author)
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Merrill, Joan (author)
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Kimberly, Robert P. (author)
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Edberg, Jeffrey C. (author)
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Ramsey-Goldman, Rosalind (author)
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Petri, Michelle (author)
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Reveille, John D. (author)
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Alarcón, Graciela S. (author)
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Vilá, Luis M. (author)
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- Alarcón-Riquelme, Marta E. (author)
- Uppsala universitet,Institutionen för genetik och patologi
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James, Judith A. (author)
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Vyse, Timothy J. (author)
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Moser, Kathy L. (author)
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Gaffney, Patrick M. (author)
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Gilkeson, Gary S. (author)
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Harley, John B. (author)
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Sawalha, Amr H. (author)
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(creator_code:org_t)
- Wiley, 2009
- 2009
- English.
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In: Arthritis and Rheumatism. - : Wiley. - 0004-3591 .- 1529-0131. ; 60:4, s. 1076-1084
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- OBJECTIVE: Both genetic and epigenetic factors play an important role in the pathogenesis of lupus. The aim of this study was to examine methyl-CpG-binding protein 2 gene (MECP2) polymorphisms in a large cohort of patients with lupus and control subjects, and to determine the functional consequences of the lupus-associated MECP2 haplotype. METHODS: We genotyped 18 single-nucleotide polymorphisms within MECP2, located on chromosome Xq28, in a large cohort of patients with lupus and control subjects of European descent. We studied the functional effects of the lupus-associated MECP2 haplotype by determining gene expression profiles in B cell lines in female lupus patients with and those without the lupus-associated MECP2 risk haplotype. RESULTS: We confirmed, replicated, and extended the genetic association between lupus and genetic markers within MECP2 in a large independent cohort of lupus patients and control subjects of European descent (odds ratio 1.35, P = 6.65 x 10(-11)). MECP2 is a dichotomous transcription regulator that either activates or represses gene expression. We identified 128 genes that are differentially expressed in lupus patients with the disease-associated MECP2 haplotype; most ( approximately 81%) were up-regulated. Genes that were up-regulated had significantly more CpG islands in their promoter regions compared with genes that were down-regulated. Gene ontology analysis using the differentially expressed genes revealed significant association with epigenetic regulatory mechanisms, suggesting that these genes are targets for MECP2 regulation in B cells. Furthermore, at least 13 of the 104 up-regulated genes are regulated by interferon. The disease-risk MECP2 haplotype was associated with increased expression of the MECP2 transcription coactivator CREB1 and decreased expression of the corepressor histone deacetylase 1. CONCLUSION: Polymorphism in the MECP2 locus is associated with lupus and, at least in part, contributes to the interferon signature observed in lupus patients.
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- MEDICIN
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- By the author/editor
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Webb, Ryan
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Wren, Jonathan D ...
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Jeffries, Matloc ...
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Kelly, Jennifer ...
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Kaufman, Kenneth ...
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Tang, Yuhong
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show more...
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Frank, Mark Bart ...
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Merrill, Joan
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Kimberly, Robert ...
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Edberg, Jeffrey ...
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Ramsey-Goldman, ...
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Petri, Michelle
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Reveille, John D ...
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Alarcón, Graciel ...
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Vilá, Luis M.
-
Alarcón-Riquelme ...
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James, Judith A.
-
Vyse, Timothy J.
-
Moser, Kathy L.
-
Gaffney, Patrick ...
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Gilkeson, Gary S ...
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Harley, John B.
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Sawalha, Amr H.
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show less...
- Articles in the publication
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Arthritis and Rh ...
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Uppsala University