Sökning: WFRF:(van Wieringen Tijs 1979 )
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Opposite effects of...
Opposite effects of PDGF-BB and prostaglandin E(1) on cell-motility related processes are paralleled by modifications of distinct actin-binding proteins
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- van Wieringen, Tijs, 1979- (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Rubin
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- Kimani, Stanley G. (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Hultgard-Ekwall, Anna-Karin (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Forsberg, Jens (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Reyhani, Vahid (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Engström, Åke (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Rubin, Kristofer (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- Engelska.
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Ingår i: Experimental Cell Research. - : Elsevier BV. - 0014-4827 .- 1090-2422. ; 315:10, s. 1745-1758
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Prostaglandin E(1) (PGE(1)) lowers dermal interstitial fluid pressure (IFP) in vivo and inhibits fibroblast-mediated collagen gel contraction in vitro. PDGF-BB, in contrast, stimulates contraction and normalizes IFP lowered as a result of anaphylaxis. Human diploid AG1518 fibroblasts expressed EP2, EP3 and IP prostaglandin receptors. The inhibitory effect of PGE(1) on contraction depended on cAMP. Short-term stimulation with PDGF-BB transiently induced formation of actin-containing membrane and circular ruffles and breakdown of stress fibers. PGE(1) had no effect on stress fibers nor did it modulate the effects of PDGF-BB. PGE(1) alone or in combination with PDGF-BB inhibited initial adhesion and spreading to collagen. PDGF-BB had no effect on adhesion but stimulated cell spreading. Two-dimensional gel electrophoresis and MALDI TOF analyses of SDS/Triton X-100-soluble proteins revealed changes in migration pattern of actin-binding proteins. Interestingly, PDGF-BB and PGE(1) affected both similar and different sets of actin-binding proteins. PDGF-BB and PGE(1) did not trans-modulate their respective effects on actin-binding proteins, cytoskeletal organization or initial adhesion. Our data show that PDGF-BB stimulates actin cytoskeleton dynamics, whereas PGE(1) inhibits processes dependent on cytoskeletal motor functions. We suggest that these different activities may partly explain the contrasting effects of PGE(1) and PDGF-BB on contraction and IFP.
Nyckelord
- Caldesmon
- Collagen gel contraction
- Cytoskeleton
- Extracellular matrix
- ERK
- Fibroblast
- Interstitial fluid pressure
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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