A novel conserved RNA-binding domain protein, RBD-1, is essential for ribosome biogenesis

• Synthesis of the ribosomal subunits from pre-rRNA requires a large number of trans-acting proteins and small nucleolar ribonucleoprotein particles to execute base modifications, RNA cleavages, and structural rearrangements. We have characterized a novel protein, RNA-binding domain-1 (RBD-1), that is involved in ribosome biogenesis. This protein contains six consensus RNA-binding domains and is conserved as to sequence, domain organization, and cellular location from yeast to human. RBD-1 is essential in Caenorhabditis elegans. In the dipteran Chironomus tentans, RBD-1 (Ct-RBD-1) binds pre-rRNA in vitro and anti-Ct-RBD-1 antibodies repress pre-rRNA processing in vivo. Ct-RBD-1 is mainly located in the nucleolus in an RNA polymerase I transcription-dependent manner, but it is also present in discrete foci in the interchromatin and in the cytoplasm. In cytoplasmic extracts, 20-30% of Ct-RBD-1 is associated with ribosomes and, preferentially, with the 40S ribosomal subunit. Our data suggest that RBD-1 plays a role in structurally coordinating pre-rRNA during ribosome biogenesis and that this function is conserved in all eukaryotes.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Animals

Caenorhabditis elegans/genetics/metabolism

Caenorhabditis elegans Proteins/genetics/*metabolism

Cell Nucleolus/metabolism

Chironomidae/anatomy & histology/genetics/*metabolism

Consensus Sequence

Fungal Proteins/genetics/metabolism

Hela Cells

Humans

Insect Proteins/genetics/metabolism

Mitosis/physiology

Nuclear Proteins/genetics/*metabolism

Protein Structure; Tertiary

RNA Interference

RNA Polymerase I/metabolism

RNA Processing; Post-Transcriptional

RNA; Ribosomal/metabolism

RNA-Binding Proteins/genetics/*metabolism

Ribonucleoproteins/metabolism

Ribosomes/*metabolism

Salivary Glands/cytology/metabolism

Transcription; Genetic

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)