Differential tissue-specific protein markers of vaginal carcinoma

The objective was to identify proteins differentially expressed in vaginal cancer to elucidate relevant cancer-related proteins. A total of 16 fresh-frozen tissue biopsies, consisting of 5 biopsies from normal vaginal epithelium, 6 from primary vaginal carcinomas and 5 from primary cervical carcinomas, were analysed using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry. Of the 43 proteins identified with significant alterations in protein expression between non-tumourous and tumourous tissue, 26 were upregulated and 17 were downregulated. Some were similarly altered in vaginal and cervical carcinoma, including cytoskeletal proteins, tumour suppressor proteins, oncoproteins implicated in apoptosis and proteins in the ubiquitin-proteasome pathway. Three proteins were uniquely altered in vaginal carcinoma (DDX48, erbB3-binding protein and biliverdin reductase) and five in cervical carcinoma (peroxiredoxin 2, annexin A2, sarcomeric tropomyosin kappa, human ribonuclease inhibitor and prolyl-4-hydrolase beta). The identified proteins imply involvement of multiple different cellular pathways in the carcinogenesis of vaginal carcinoma. Similar protein alterations were found between vaginal and cervical carcinoma suggesting common tumourigenesis. However, the expression level of some of these proteins markedly differs among the three tissue specimens indicating that they might be useful molecular markers.

Alaiya, Ayodele A.Karolinska Institutet (författare)
Becker, SusanneUnit of Cancer Proteomics, Department of Oncology and Pathology, Karolinska Institute and Hospital, SE-171 76 Stockholm, Sweden (författare)
Lomnytska, MartaKarolinska Institutet(Swepub:uu)marlo829 (författare)
Schedvins, KjellDepartment of Gynaecology, Karolinska University Hospital, SE-171 76 Stockholm, Sweden (författare)
Steinberg, WinfriedInstitution of Cytological Diagnosis (Kloster-Paradiese), Im Stiftsfeld, 159494 Soest, Germany (författare)
Hellström, Ann-CathrinDepartment of Gynaecologic Oncology, Radiumhemmet, Karolinska University Hospital, SE-171 76 Stockholm, Sweden (författare)
Andersson, SoniaKarolinska Institutet (författare)
Hellman, UlfUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)ulfhm (författare)
Auer, GertKarolinska Institutet (författare)
Karolinska InstitutetUnit of Cancer Proteomics, Department of Oncology and Pathology, Karolinska Institute and Hospital, SE-171 76 Stockholm, Sweden (creator_code:org_t)

Ingår i:British Journal of Cancer: Cancer Research UK100:8, s. 1303-13140007-09201532-1827

Av författaren/redakt...: Hellman, Kristin ...; Alaiya, Ayodele ...; Becker, Susanne; Lomnytska, Marta; Schedvins, Kjell; Steinberg, Winfr ...; visa fler...; Hellström, Ann-C ...; Andersson, Sonia; Hellman, Ulf; Auer, Gert; visa färre...

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