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In vitro deadenylat...
In vitro deadenylation of mammalian mRNA by a HeLa cell 3' exonuclease
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- Åström, Jonas (author)
- Uppsala universitet,Medicinsk genetik
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- Åström, Anders (author)
- Uppsala universitet,Medicinsk genetik
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- Virtanen, Anders (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Medicinsk genetik
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(creator_code:org_t)
- 1991
- 1991
- English.
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In: EMBO Journal. - 0261-4189 .- 1460-2075. ; 10:10, s. 3067-3071
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- We have identified a 3' exonuclease in HeLa cell extracts which deadenylates mammalian mRNA and leaves the mRNA body intact after poly(A) removal. Only homopolymeric adenosine tails located at the 3' end were efficiently removed by the exonuclease. The poly(A) removing activity did not require any specific sequences in the mRNA body either for poly(A) removal or for accumulation of the deadenylated mRNA. We conclude that the poly(A) removing activity is a 3' exonuclease since (i) reaction intermediates gradually lose the poly(A) tail, (ii) degradation is prevented by the presence of a cordycepin residue at the 3' end and (iii) RNAs having internally located poly(A) stretches are poor substrates for degradation. The possible involvement of the poly(A) removing enzyme in regulating mRNA translation and stability is discussed.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Keyword
- Medical genetics
- Medicinsk genetik
Publication and Content Type
- ref (subject category)
- art (subject category)
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