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Low-coverage pyrose...
Low-coverage pyrosequencing reveals recombination and run-off replication in Bartonella henselae strains
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- Guy, Lionel (författare)
- Uppsala universitet,Molekylär evolution,Molecular Evolution
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- Nystedt, Björn (författare)
- Molecular Evolution
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- Sun, Yu (författare)
- Uppsala universitet,Molekylär evolution,Molecular Evolution
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visa fler...
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- Berglund, Eva C. (författare)
- Uppsala universitet,Molekylär evolution,Molecular Evolution
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- Graf, Alexander (författare)
- Uppsala universitet,Molekylär evolution,Molecular Evolution
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- Zhoupeng, Xie (författare)
- Uppsala universitet,Molekylär evolution,Molecular Evolution
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- Näslund, Kristina (författare)
- Uppsala universitet,Molekylär evolution,Molecular Evolution
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- Andersson, Siv G.E. (författare)
- Uppsala universitet,Molekylär evolution,Molecular Evolution
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Bartonella henselae is a natural intracellular colonizer of cats, and is transferred by blood-sucking insect vectors. It is also an opportunistic human pathogen. Two strains of B. henselae, thought to be representative of the diversity of the species, were selected for low-coverage 454 sequencing. The comparison of these two strains to the published Houston-1 reveals very high nucleotide identity and low substitution and recombination, with the remarkable exception of phages and host-interaction genes such as type IV and V secretion systems. Among the few variable genes of unknown function, BH14680, an alpha-Proteobacteria-specific gene, shows faster evolution in Bartonella compared to other alpha-Proteobacteria. Its 5’ end, which is likely coding for a domain exposed extracellularly, is under positive or very relaxed selection, and might be involved in host-interaction processes. Finally, we show that a simple genome coverage analysis reveal major genomic events such as duplications and unusual replication modes, such as the run-off replication. The latter, combined with a gene transfer agent, is thought to be a novel way to increase substitution and recombination frequencies. An extensive analysis of all bacterial pyrosequencing projects showed that it is probably Bartonella-specific.
Ämnesord
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Nyckelord
- pathogen
- recombination
- run-off replication
- phage
- gene transfer agent
- pyrosequencing
- evolution
- Bioinformatics
- Bioinformatik
- Evolutionary Genetics
- evolutionär genetik
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)