Towards Automated TEM for Virus Diagnostics : Segmentation of Grid Squares and Detection of Regions of Interest

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• When searching for viruses in an electron microscope thesample grid constitutes an enormous search area. Here, we present methodsfor automating the image acquisition process for an automatic virusdiagnostic application. The methods constitute a multi resolution approachwhere we first identify the grid squares and rate individual gridsquares based on content in a grid overview image and then detect regionsof interest in higher resolution images of good grid squares. Our methodsare designed to mimic the actions of a virus TEM expert manually navigatingthe microscope and they are also compared to the expert’s performance.Integrating the proposed methods with the microscope wouldreduce the search area by more than 99.99% and it would also removethe need for an expert to perform the virus search by the microscope.

Ämnesord

NATURVETENSKAP  -- Data- och informationsvetenskap -- Datorseende och robotik (hsv//swe)

NATURAL SCIENCES  -- Computer and Information Sciences -- Computer Vision and Robotics (hsv//eng)

NATURVETENSKAP  -- Data- och informationsvetenskap -- Datavetenskap (hsv//swe)

NATURAL SCIENCES  -- Computer and Information Sciences -- Computer Sciences (hsv//eng)

Nyckelord

TEM

virus diagnostics

automatic image acquisition

Image analysis

Bildanalys

Computerized Image Analysis

Datoriserad bildanalys

Publikations- och innehållstyp

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