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Cytochrome P450 1D1 :
Cytochrome P450 1D1 : A novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD
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Goldstone, J. V. (författare)
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- Jönsson, Maria, 1961- (författare)
- Uppsala universitet,Ekotoxikologi
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Behrendt, L. (författare)
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Woodin, B. R. (författare)
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Jenny, M. J. (författare)
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Nelson, D. R. (författare)
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Stegeman, J. J. (författare)
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(creator_code:org_t)
- Amsterdam : Elsevier inc. 2009
- 2009
- Engelska.
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Ingår i: Archives of Biochemistry and Biophysics. - Amsterdam : Elsevier inc.. - 0003-9861 .- 1096-0384. ; 482:1-2, s. 7-16
- Relaterad länk:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Enzymes in the cytochrome P450 1 family oxidize many common environmental toxicants. We identified a new CYP1, termed CYP1D1, in zebrafish. Phylogenetically, CYP1D1 is paralogous to CYP1A and the two share 45% amino acid identity and similar gene structure. In adult zebrafish, CYP1D1 is most highlyexpressed in liver and is relatively highly expressed in brain. CYP1D1 transcript levels were higher at 9 h post-fertilization than at later developmental times. Treatment of zebrafish with potent aryl hydrocarbon receptor (AHR) agonists (3,3',4,4',5-pentachlorobiphenyl or 2,3,7,8-tetrachlorodibenzo-p-dioxin) did not induce CYP1D1 transcript expression. Morpholino oligonucleotide knockdown of AHR2, which mediates induction of other CYP1s, did not affect CYP1D1 expression. Zebrafish CYP1D1 heterologously expressed in yeast exhibited ethoxyresorufin- and methoxyresorufin-O-dealkylase activities. Antibodies against a CYP1D1 peptide specifically detected a single electrophoretically-resolved protein band in zebrafish liver microsomes, distinct from CYP1A. CYP1D1 in zebrafish is a CYP1A-like gene that could have metabolic functions targeting endogenous compounds.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- zebrafish
- CYP1D1
- embryo
- development
- gene expression
- Molecular biology
- Molekylärbiologi
- Biochemistry
- biokemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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