The role of iron in the proliferation of Drosophila l(2)mbn cells

• Iron is essential for life and is needed for cell proliferation and cell cycle progression. Iron deprivation induces cell cycle arrest and finally apoptosis. The Drosophila tumorous larval hemocyte cell line l(2)mbn was used to study the sensitivity and cellular response to iron deprivation through the chelator desferrioxamine (DFO). At a concentration of 10 mM DFO or more the proliferation was inhibited reversibly, while the amount of dead cells did not increase. FACS analysis showed that the cell cycle was arrested in G1/S phase and the transcript level of cycE was decreased to less than 50% of control cells. These results show that iron chelation in this insect tumorous cell line causes a specific and coordinated cell cycle arrest.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

iron drosophila cell cycle arrest cyclin cell proliferation

Cell and molecular biology

Cell- och molekylärbiologi

Biology with specialization in Comparative Physiology

Biologi med inriktning mot jämförande fysiologi

Publication and Content Type

ref (subject category)

art (subject category)