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Angiomotin regulate...
Angiomotin regulates endothelial cell migration during embryonic angiogenesis
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- Aase, Karin (författare)
- Department of Oncology-Pathology, Cancer Center Karolinska, Karolinska Institutet, SE-17176 Stockholm, Sweden
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- Ernkvist, Mira (författare)
- Department of Oncology-Pathology, Cancer Center Karolinska, Karolinska Institutet, SE-17176 Stockholm, Sweden
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- Ebarasi, Lwaki (författare)
- Karolinska Institutet
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- Jakobsson, Lars (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Majumdar, Arindam (författare)
- Karolinska Institutet
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- Yi, Chunling (författare)
- Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
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- Birot, Olivier (författare)
- Department of Oncology-Pathology, Cancer Center Karolinska, Karolinska Institutet, SE-17176 Stockholm, Sweden
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- Ming, Yue (författare)
- Department of Clinical Neuroscience, Section of Ophthalmology and Vision, Karolinska Institutet, St Erik’s Hospital, SE-11284 Stockholm, Sweden
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- Kvanta, Anders (författare)
- Karolinska Institutet
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- Edholm, Dan (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Aspenström, Pontus (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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- Kissil, Joseph (författare)
- Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
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- Claesson-Welsh, Lena (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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- Shimono, Akihiko (författare)
- Vertebrate Body Plan, Center for Developmental Biology, RIKEN Kobe, Chuou-ku, Kobe 650-0047, Japan
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- Holmgren, Lars (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2007-08-15
- 2007
- Engelska.
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Ingår i: Genes & Development. - : Cold Spring Harbor Laboratory. - 0890-9369 .- 1549-5477. ; 21:16, s. 2055-2068
- Relaterad länk:
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http://genesdev.cshl...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The development of the embryonic vascular system into a highly ordered network requires precise control over the migration and branching of endothelial cells (ECs). We have previously identified angiomotin (Amot) as a receptor for the angiogenesis inhibitor angiostatin. Furthermore, DNA vaccination targeting Amot inhibits angiogenesis and tumor growth. However, little is known regarding the role of Amot in physiological angiogenesis. We therefore investigated the role of Amot in embryonic neovascularization during zebrafish and mouse embryogenesis. Here we report that knockdown of Amot in zebrafish reduced the number of filopodia of endothelial tip cells and severely impaired the migration of intersegmental vessels. We further show that 75% of Amot knockout mice die between embryonic day 11 (E11) and E11.5 and exhibit severe vascular insufficiency in the intersomitic region as well as dilated vessels in the brain. Furthermore, using ECs differentiated from embryonic stem (ES) cells, we demonstrate that Amot-deficient cells have intact response to vascular endothelial growth factor (VEGF) in regard to differentiation and proliferation. However, the chemotactic response to VEGF was abolished in Amot-deficient cells. We provide evidence that Amot is important for endothelial polarization during migration and that Amot controls Rac1 activity in endothelial and epithelial cells. Our data demonstrate a critical role for Amot during vascular patterning and endothelial polarization.
Nyckelord
- Angiostatin
- neovascularization
- embryogenesis
- GTPase
- chemotaxis
- transgenic
- zebrafish
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Aase, Karin
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Ernkvist, Mira
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Ebarasi, Lwaki
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Jakobsson, Lars
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Majumdar, Arinda ...
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Yi, Chunling
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visa fler...
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Birot, Olivier
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Ming, Yue
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Kvanta, Anders
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Edholm, Dan
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Aspenström, Pont ...
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Kissil, Joseph
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Claesson-Welsh, ...
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Shimono, Akihiko
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Holmgren, Lars
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- Artiklar i publikationen
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Genes & Developm ...
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet