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Enhanced neuronal d...
Enhanced neuronal differentiation in a three-dimensional collagen-hyaluronan matrix
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- Brännvall, Karin (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Bergman, Kristoffer (author)
- Uppsala universitet,Institutionen för materialkemi
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- Wallenquist, Ulrika (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Svahn, Stefan (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Bowden, Tim (author)
- Uppsala universitet,Institutionen för materialkemi
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- Hilborn, Jöns (author)
- Uppsala universitet,Institutionen för materialkemi
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- Forsberg-Nilsson, Karin (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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(creator_code:org_t)
- Wiley, 2007
- 2007
- English.
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In: Journal of Neuroscience Research. - : Wiley. - 0360-4012 .- 1097-4547. ; 85:10, s. 2138-2146
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Efficient 3D cell systems for neuronal induction are needed for future use in tissue regeneration. In this study, we have characterized the ability of neural stem/progenitor cells (NS/PC) to survive, proliferate, and differentiate in a collagen type I-hyaluronan scaffold. Embryonic, postnatal, and adult NS/PC were seeded in the present 3D scaffold and cultured in medium containing epidermal growth factor and fibroblast growth factor-2, a condition that stimulates NS/PC proliferation. Progenitor cells from the embryonic brain had the highest proliferation rate, and adult cells the lowest, indicating a difference in mitogenic responsiveness. NS/PC from postnatal stages down-regulated nestin expression more rapidly than both embryonic and adult NS/PC, indicating a faster differentiation process. After 6 days of differentiation in the 3D scaffold, NS/PC from the postnatal brain had generated up to 70% neurons, compared with 14% in 2D. NS/PC from other ages gave rise to approximately the same proportion of neurons in 3D as in 2D (9-26% depending on the source for NS/PC). In the postnatal NS/PC cultures, the majority of III-tubulin-positive cells expressed glutamate, -aminobutyric acid, and synapsin I after 11 days of differentiation, indicating differentiation to mature neurons. Here we report that postnatal NS/PC survive, proliferate, and efficiently form synapsin I-positive neurons in a biocompatible hydrogel.
Keyword
- 3D cultures
- neural stem/progenitor cells
- hydrogel
- scaffold
- neurogenesis
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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