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Enhanced neuronal d...
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Brännvall, KarinUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
(author)
Enhanced neuronal differentiation in a three-dimensional collagen-hyaluronan matrix
- Article/chapterEnglish2007
Publisher, publication year, extent ...
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Wiley,2007
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-11683
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-11683URI
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https://doi.org/10.1002/jnr.21358DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Efficient 3D cell systems for neuronal induction are needed for future use in tissue regeneration. In this study, we have characterized the ability of neural stem/progenitor cells (NS/PC) to survive, proliferate, and differentiate in a collagen type I-hyaluronan scaffold. Embryonic, postnatal, and adult NS/PC were seeded in the present 3D scaffold and cultured in medium containing epidermal growth factor and fibroblast growth factor-2, a condition that stimulates NS/PC proliferation. Progenitor cells from the embryonic brain had the highest proliferation rate, and adult cells the lowest, indicating a difference in mitogenic responsiveness. NS/PC from postnatal stages down-regulated nestin expression more rapidly than both embryonic and adult NS/PC, indicating a faster differentiation process. After 6 days of differentiation in the 3D scaffold, NS/PC from the postnatal brain had generated up to 70% neurons, compared with 14% in 2D. NS/PC from other ages gave rise to approximately the same proportion of neurons in 3D as in 2D (9-26% depending on the source for NS/PC). In the postnatal NS/PC cultures, the majority of III-tubulin-positive cells expressed glutamate, -aminobutyric acid, and synapsin I after 11 days of differentiation, indicating differentiation to mature neurons. Here we report that postnatal NS/PC survive, proliferate, and efficiently form synapsin I-positive neurons in a biocompatible hydrogel.
Subject headings and genre
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3D cultures
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neural stem/progenitor cells
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hydrogel
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scaffold
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neurogenesis
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MEDICINE
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MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
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Bergman, KristofferUppsala universitet,Institutionen för materialkemi(Swepub:uu)krber255
(author)
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Wallenquist, UlrikaUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
(author)
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Svahn, StefanUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
(author)
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Bowden, TimUppsala universitet,Institutionen för materialkemi(Swepub:uu)tibow735
(author)
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Hilborn, JönsUppsala universitet,Institutionen för materialkemi(Swepub:uu)johil255
(author)
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Forsberg-Nilsson, KarinUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)karifors
(author)
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Uppsala universitetInstitutionen för medicinsk biokemi och mikrobiologi
(creator_code:org_t)
Related titles
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In:Journal of Neuroscience Research: Wiley85:10, s. 2138-21460360-40121097-4547
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