Expression of T cell immunoglobulin- and mucin-domain-containing molecules-1 and -3 (TIM-1 and -3) in the rat nervous and immune systems

Expression of T cell immunoglobulin- and mucin-domain-containing molecules (TIMs) can be used as T helper (Th) differentiation markers in the human and mouse. We examined the expression of TIM-1 and -3 mRNAs in rat MBP(63-88)-induced experimental autoimmune encephalomyelitis (EAE). TIM-3 expression was upregulated in the spinal cord during EAE and following antigen restimulation of the encephalitogenic TCRBV8S2+ population. Interestingly, TIM-3 expression was also detected by in situ hybridization in resident cells of the nervous system. TIM-1 was expressed in B cells but not in resident CNS cells and TIM-1 mRNA levels in spinal cord were unchanged throughout the course of EAE. These results support the notion that TIM-3 can also be used as a Th1 differentiation marker in the rat. However, expression of TIM-1 and -3 is not restricted solely to T cells and the presence of TIM-3 in resident CNS cells may indicate a role for this molecule in the interaction between the nervous and immune systems.

Lobell, AnnaUppsala universitet,Institutionen för medicinska vetenskaper,Lobell / Kämpe (author)
Lidman, OlleKarolinska Institutet (author)
Khademi, Mohsen (author)
Olsson, TomasKarolinska Institutet (author)
Piehl, FredrikKarolinska Institutet (author)
Khademi, N (author)
Uppsala universitetInstitutionen för medicinska vetenskaper (creator_code:org_t)

By the author/editor: Gielen, Alexande ...; Lobell, Anna; Lidman, Olle; Khademi, Mohsen; Olsson, Tomas; Piehl, Fredrik; show more...; Khademi, N; show less...

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