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Efforts to improve ...
Efforts to improve detection sensitivity for capillary electrophoresis coupled to atmospheric pressure photoionization mass spectrometry.
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- Axén, Jakob (author)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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- Malmström, David (author)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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Axelsson, Bengt-Olof (author)
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Petersson, Patrik (author)
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- Sjöberg, Per J. R. (author)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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(creator_code:org_t)
- John Wiley & Sons Ltd, 2010
- 2010
- English.
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In: Rapid Communications in Mass Spectrometry. - : John Wiley & Sons Ltd. - 0951-4198 .- 1097-0231. ; 24:9, s. 1260-1264
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Electrospray ionization performs best with volatile buffers. However, generally the best separation performance for capillary electrophoresis (CE) is achieved with non-volatile buffers. Hyphenation of CE with mass spectrometry (MS) utilizing atmospheric pressure photoionization (APPI) enables use of a wider range of separation buffers without compromising detection sensitivity. As APPI is considered to be mass flow sensitive, the use of a larger inner diameter separation capillary (75 microm) allows larger volumes to be injected, without decreased separation performance, thus providing improved sensitivity (approx. a factor of 10), compared to the use of a 25 microm capillary. However, nebulizing gas flow and position of capillary tip in the sprayer have to be carefully optimized to prevent excessive band broadening. Further improvement in sensitivity (approx. a factor of 2) was obtained by decreasing the distance between the sprayer and ionization region, indicating that a specially designed CE/APPI-MS interface for low flow rates will be favourable.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Analytical chemistry
- Analytisk kemi
- Analytical Chemistry
- Analytisk kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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