Application of Artificial Gel Antibodies for the Detection and Quantification of Proteins in Biological Fluids

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MARC

Ghasemzadeh, NasimUppsala universitet,Institutionen för farmaceutisk biovetenskap,Fred Nyberg (author)

Application of Artificial Gel Antibodies for the Detection and Quantification of Proteins in Biological Fluids

BookEnglish2010

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Uppsala :Acta Universitatis Upsaliensis,2010
64 s.
electronicrdacarrier

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LIBRIS-ID:oai:DiVA.org:uu-122457
ISBN:9789155478025
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122457URI

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Language:English
Summary in:English

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SwePubSwePub

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Subject category:vet swepub-contenttype
Subject category:dok swepub-publicationtype

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Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology,1651-6214 ;741

Notes

The molecular-imprinting method has previously been used for the synthesis of artificial gel antibodies, highly selective for various proteins. In present study, we have synthesized artificial gel antibodies against haemoglobin, albumin and different forms of growth hormone with the aim to develop a simple and rapid procedure to measure the concentration of these protein biomarkers in samples of clinical interest. A spectrophotometric method was developed to design a standard curve in the form of a straight line, whereby the true absorption (not the recorded “apparent” absorption) was plotted against a known protein concentration. The procedure, applied to quantitative analysis of albumin in human plasma and cerebrospinal fluid (CSF) from patients with ALS, indicated that the concentration of this protein was significantly enhanced in CSF from patients with amyotrophic lateral sclerosis (ALS), compared to control samples. A low level of albumin was observed in plasma from ALS patients compared to controls. Additionally, free zone electrophoresis was employed to detect human growth hormone (GH) activity in hormone preparations purified from human pituitaries. We have successfully synthesized antibodies capable of discriminating between dimeric and monomeric GH in samples of clinical origin. To quantify these proteins a calibration curve has been designed, i.e. a plot of the electrophoretic mobility of the complex GH/gel antibody against the protein concentration in the sample, for instance serum or CSF. This method was also employed for qualitative and quantitative determinations of Somatropin, a non-glycosylated GH and glycosylated-GH in a body liquid. Our results indicate that by this technique one can “fish out” with high accuracy various proteins from both body fluids containing a great number of other proteins. It might well apply also to biomarker proteins for other diseases.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Läkemedelskemi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Medicinal Chemistry hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Farmaceutiska vetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Pharmaceutical Sciences hsv//eng
Pharmaceutical chemistry
Farmaceutisk kemi
Pharmaceutical biochemistry
Farmaceutisk biokemi
Farmaceutisk vetenskap
Pharmaceutical Science

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Nyberg, Fred,Prof.Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Avdelningen för biologisk beroendeforskning (thesis advisor)
Desiderio, Dom M.,Prof.Charles B. Stout Neuroscience Mass Spectrometry Laboratory (opponent)
Uppsala universitetInstitutionen för farmaceutisk biovetenskap (creator_code:org_t)

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