The sensitivity of hyaluronan analysis of pleural fluid from patients with malignant mesothelioma and a comparison of different methods

LIBRIS-ID:oai:DiVA.org:uu-122710
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122710URI
https://doi.org/10.1002/1097-0142(19940301)73:5<1406::AID-CNCR2820730515>3.0.CO;2-IDOI

BACKGROUND: Hyaluronan may be used as a marker for malignant mesothelioma, thus indicating its mesodermal origin. METHODS: The sensitivity as a diagnostic test of three different methods for hyaluronan analyses of pleural fluid was examined in patients with biopsy-verified malignant pleural mesothelioma. RESULTS: A quantitative high-performance liquid-chromatography (HPLC) method was performed on fluids from 43 patients. Using a cutoff level of 100 mg/l, higher levels were noted in 30 (70%) patients, with a median value of 220 mg/l (mean, 560 mg/l; range, 20-6600 mg/l). An identical median value (220 mg/l) was obtained with a radioassay method when simultaneously performed on paired samples from 21 patients (correlation coefficient, 0.91). A qualitative precipitation test using 0.5% cetylpyridinium chloride combined with a quantitative viscosimetric method was significantly less sensitive (P < 0.01). CONCLUSION: Hyaluronan analyses is beneficial in distinguishing malignant mesothelioma if methods such as the evaluated HPLC or radioassay with a sensitivity of 70% toward mesothelioma are used and other known causes of elevated content are considered.

Thylen, Anders (author)
Lindqvist, UllaUppsala universitet,Institutionen för medicinska vetenskaper,Reumatologi (author)
Hjerpe, Anders (author)
Uppsala universitetInstitutionen för medicinska vetenskaper (creator_code:org_t)

By the author/editor: Mårtensson, Gunn ...; Thylen, Anders; Lindqvist, Ulla; Hjerpe, Anders

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