Search: WFRF:(Gustafsdottir Sigrun) >
Sensitive plasma pr...
Sensitive plasma protein analysis by microparticle-based proximity ligation assays
-
- Darmanis, Spyros (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Nong, Rachel Yuan (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Hammond, Maria (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
show more...
-
- Gu, Jijuan (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Alderborn, Anders (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Vänelid, Johan (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Siegbahn, Agneta (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
- Gustafsdottir, Sigrun (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Ericsson, Olle (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Landegren, Ulf (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Kamali-Moghaddam, Masood (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
show less...
-
(creator_code:org_t)
- 2010
- 2010
- English.
-
In: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 9:2, s. 327-335
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Detection of proteins released in the bloodstream from tissues damaged by disease can promote early detection of pathological conditions, differential diagnostics, and follow-up of therapy. Despite these prospects and a plethora of candidate biomarkers, efforts in recent years to establish new protein diagnostic assays have met with limited success. One important limiting factor has been the challenge of detecting proteins present at trace levels in complex bodily fluids. To achieve robust, sensitive, and specific detection, we have developed a microparticle-based solid-phase proximity ligation assay, dependent on simultaneous recognition of target proteins by three antibody molecules for added specificity. After capture on a microparticle, solid-phase pairs of proximity probes are added followed by washes, enabling detection and identification of rare protein molecules in blood while consuming small amounts of sample. We demonstrate that single polyclonal antibody preparations raised against target proteins of interest can be readily used to establish assays where detection depends on target recognition by three individual antibody molecules, recognizing separate epitopes. The assay was compared with state-of-the-art sandwich ELISAs for detection of vascular endothelial growth factor, interleukin-8 and interleukin-6, and it was found to be superior both with regard to dynamic range and minimal numbers of molecules detected. Furthermore, the assays exhibited excellent performance in undiluted plasma and serum as well as in whole blood, producing comparable results for nine different antigens. We thus show that solid-phase proximity ligation assay is suitable for validation of a variety of protein biomarkers over broad dynamic ranges in clinical samples.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Darmanis, Spyros
-
Nong, Rachel Yua ...
-
Hammond, Maria
-
Gu, Jijuan
-
Alderborn, Ander ...
-
Vänelid, Johan
-
show more...
-
Siegbahn, Agneta
-
Gustafsdottir, S ...
-
Ericsson, Olle
-
Landegren, Ulf
-
Kamali-Moghaddam ...
-
show less...
- Articles in the publication
-
Molecular & Cell ...
- By the university
-
Uppsala University