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T cell specific ada...
T cell specific adapter protein (TSAd) interacts with Tec kinase ITK to promote CXCL12 induced migration of human and murine T cells
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Berge, Tone (författare)
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Sundvold-Gjerstad, Vibeke (författare)
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Granum, Stine (författare)
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Andersen, Thorny C. (författare)
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Holthe, Gunn B. (författare)
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- Claesson-Welsh, Lena (författare)
- Uppsala universitet,Institutionen för genetik och patologi
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Andreotti, Amy H. (författare)
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Inngjerdingen, Marit (författare)
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Spurkland, Anne (författare)
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(creator_code:org_t)
- 2010-03-18
- 2010
- Engelska.
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Ingår i: PloS one. - : Public Library of Science (PLoS). - 1932-6203. ; 5:3, s. e9761-
- Relaterad länk:
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- BACKGROUND: The chemokine CXCL12/SDF-1alpha interacts with its G-protein coupled receptor CXCR4 to induce migration of lymphoid and endothelial cells. T cell specific adapter protein (TSAd) has been found to promote migration of Jurkat T cells through interaction with the G protein beta subunit. However, the molecular mechanisms for how TSAd influences cellular migration have not been characterized in detail. PRINCIPAL FINDINGS: We show that TSAd is required for tyrosine phosphorylation of the Lck substrate IL2-inducible T cell kinase (Itk). Presence of Itk Y511 was necessary to boost TSAd's effect on CXCL12 induced migration of Jurkat T cells. In addition, TSAd's ability to promote CXCL12-induced actin polymerization and migration of Jurkat T lymphocytes was dependent on the Itk-interaction site in the proline-rich region of TSAd. Furthermore, TSAd-deficient murine thymocytes failed to respond to CXCL12 with increased Itk phosphorylation, and displayed reduced actin polymerization and cell migration responses. CONCLUSION: We propose that TSAd, through its interaction with both Itk and Lck, primes Itk for Lck mediated phosphorylation and thereby regulates CXCL12 induced T cell migration and actin cytoskeleton rearrangements.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Nyckelord
- Cell biology
- Cellbiologi
- Molecular medicine (genetics and pathology)
- Molekylär medicin (genetik och patologi)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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PloS one
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