Quantification and Localization of Colocalization

• This paper presents a comparison of two well known and commonly used methods for quantification of color colocalization in fluorescence microscopy image data. We also propose a new method based on a modified spectral decomposition borrowed from the field of remote sensing. Quantification and localization of colocalized pixels using modified spectral decomposition proved to be more robust than previous method when tested on a data set of artificial images with increasing levels of noise. The proposed method was also tested on a data set consisting of 16 color channels, showing that it is easily extendable to colocalization problems in more than two color dimensions.

Ämnesord

NATURVETENSKAP  -- Data- och informationsvetenskap -- Datorseende och robotik (hsv//swe)

NATURAL SCIENCES  -- Computer and Information Sciences -- Computer Vision and Robotics (hsv//eng)

Nyckelord

image analysis

fluorescence microscopy

colocalization

spectral decomposition

Image analysis

Bildanalys

Computerized Image Analysis

datoriserad bildanalys

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