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- Allalou, AminUppsala universitet,Centrum för bildanalys,Datoriserad bildanalys (author)
Image Based Measurements of Single Cell mtDNA Mutation Load
- Article/chapterEnglish2007
Publisher, publication year, extent ...
- Berlin, Heidelberg :Springer Berlin Heidelberg,2007
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-12453
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-12453URI
- https://doi.org/10.1007/978-3-540-73040-8DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- Cell cultures as well as cells in tissue always display a certain degree of variability, and measurements based on cell averages will miss important information contained in a heterogeneous population. This paper presents automated methods for image based measurements of mitochondiral DNA (mtDNA) mutations in individual cells. The mitochondria are present in the cell’s cytoplasm, and each cytoplasm has to be delineated. Three different methods for segmentation of cytoplasms are compared and it is shown that automated cytoplasmic delineation can be performed 30 times faster than manual delineation, with an accuracy as high as 87%. The final image based measurements of mitochondrial mutation load are also compared to, and show high agreement with, measurements made using biochemical techniques.
Subject headings and genre
- NATURVETENSKAP Data- och informationsvetenskap Datorseende och robotik hsv//swe
- NATURAL SCIENCES Computer and Information Sciences Computer Vision and Robotics hsv//eng
- single cell analysis
- cytoplasm segmentation
- mitochondrial DNA
- image cytometry
- Image analysis
- Bildanalys
Added entries (persons, corporate bodies, meetings, titles ...)
- van de Rijke, Frans M. (author)
- Jahangir Tafrechi, Roos (author)
- Raap, Anton K. (author)
- Wählby, CarolinaUppsala universitet,Centrum för bildanalys(Swepub:uu)cli05194 (author)
- Uppsala universitetCentrum för bildanalys (creator_code:org_t)
Related titles
- In:Image Analysis, ProceedingsBerlin, Heidelberg : Springer Berlin Heidelberg, s. 631-6409783540730392
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