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Steroid profiles in...
Steroid profiles in ovarian follicular fluid in women with and without polycystic ovary syndrome, analyzed by liquid chromatography-tandem mass spectrometry.
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- Naessén, Tord (author)
- Uppsala universitet,Institutionen för kvinnors och barns hälsa,Gynekologisk endokrinologi/Naessén
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- Kushnir, Mark M. (author)
- Uppsala universitet,Analytisk kemi
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- Chaika, Andrey (author)
- Donetsk State Medical University, Ukraine
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- Nosenko, Jelena (author)
- Donetsk State Medical University, Ukraine
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- Mogilevkina, Iryna (author)
- Donetsk State Medical University, Ukraine
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- Rockwood, Alan L. (author)
- ARUP Institute for Clinical and Experimental Pathology, Utah
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- Carlström, Kjell (author)
- Karolinska Institute
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- Bergquist, Jonas (author)
- Uppsala universitet,Analytisk kemi
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- Kirilovas, Dmitrijus (author)
- Uppsala universitet,Institutionen för kvinnors och barns hälsa,Gynekologisk endokrinologi/Naessén
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(creator_code:org_t)
- Elsevier BV, 2010
- 2010
- English.
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In: Fertility and Sterility. - : Elsevier BV. - 0015-0282 .- 1556-5653. ; 94:6, s. 2228-2233
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Subject headings
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- OBJECTIVE To compare steroid concentrations and steroid product-to-precursor ratios in ovarian follicular fluid (FF) from women with polycystic ovary syndrome (PCOS) and from regularly menstruating women in their early follicular phase, using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Polycystic ovary syndrome involves abnormal regulation of the steroidogenic enzymes, leading to arrest of follicle development. DESIGN Case-control study. SETTING University hospital clinic. PATIENT(S) Follicular fluid from size-matched ovarian follicles (5-8 mm) in 27 nonstimulated women with PCOS and in 21 women without PCOS was sampled. Thirteen steroids were quantitated from 40 muL of FF, using LC-MS/MS. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) Concentrations of steroids in the FF and product-to-precursor ratios (enzyme activity) were compared between the groups. RESULT(S) In women with PCOS, ovarian FF contained higher concentrations of individual and total androgens, lower individual and total estrogens (E), and a lower total E-to-androgen ratio, compared with regularly menstruating women. The product-to-precursor concentration ratios indicated higher CYP17-linked and lower CYP19-linked (aromatase) enzyme activity. Receiver operating characteristic plots indicated the early CYP17 step (17-OH5P/5P) being highly important for the prevalence of PCOS (c = 0.95). CONCLUSION(S) The women with PCOS had higher ovarian CYP17-linked and lower CYP19-linked (aromatase) enzyme activity, confirming previous data. Multiple steroid assessments from minute volumes including FF from nonstimulated ovaries, using LC-MS/MS, might be useful in research, clinical endocrinology, and in IVF.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- Androgens
- CYP17
- CYP19
- estrogens
- follicular fluid
- LC-MS/MS
- mass spectrometry
- PCOS
- Polycystic ovary syndrome
- steroid hormones
- TECHNOLOGY
- TEKNIKVETENSKAP
- Obstetrics and women's diseases
- Obstetrik och kvinnosjukdomar
- Chemistry
- Kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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