Colorimetric Protein Sensing by Controlled Assembly of Gold Nanoparticles Functionalized with Synthetic Receptors

Aili, Daniel,1977-Linköpings universitet,Molekylär fysik,Tekniska fakulteten (author)

A novel strategy is described for the colorimetric sensing of proteins, based on polypeptide-functionalized gold nanoparticles. Recognition is accomplished using a polypeptide sensor scaffold designed to specifically bind to the model analyte, human carbonic anhydrase II (HCAII). The extent of particle aggregation, induced by the Zn2+-triggered dimerization and folding of a second polypeptide also present on the surface of the gold nanoparticle, gives a readily detectable colorimetric shift that is dependent on the concentration of the target protein. In the absence of HCAII, particle aggregation results in a major redshift of the plasmon peak, whereas analyte binding prevented the formation of dense aggregates, significantly reducing the magnitude of the redshift. The versatility of the technique is demonstrated using a second model system based on the recognition of a peptide sequence from the tobacco mosaic virus coat protein (TMVP) by a recombinant antibody fragment (Fab57P). Concentrations down to approximate to 10 nM and approximate to 25 nM are detected for HCAII and Fab57P, respectively. This strategy is proposed as a generic platform for robust and specific protein analysis that can be further developed to monitor a wide range of target proteins.

Selegård, RobertLinköpings universitet,Molekylär fysik,Tekniska fakulteten(Swepub:liu)robse34 (author)
Baltzer, LarsUppsala universitet,Institutionen för biokemi och organisk kemi,Division of Organic Chemistry, Department of Biochemistry and Organic Chemistry, BMC, Box 576, Uppsala University, SE-751 23 Uppsala, Sweden(Swepub:uu)labal087 (author)
Enander, KarinLinköpings universitet,Molekylär fysik,Tekniska fakulteten(Swepub:liu)karen14 (author)
Liedberg, BoLinköpings universitet,Molekylär fysik,Tekniska fakulteten(Swepub:liu)boli31 (author)
Linköpings universitetMolekylär fysik (creator_code:org_t)

By the author/editor: Aili, Daniel, 19 ...; Selegård, Robert; Baltzer, Lars; Enander, Karin; Liedberg, Bo

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