Characterization of the neuropeptide Y system in the frog Silurana tropicalis (Pipidae): three peptides and six receptor subtypes

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Sundström, GörelUppsala universitet,Institutionen för neurovetenskap,Larhammar (author)

Characterization of the neuropeptide Y system in the frog Silurana tropicalis (Pipidae) : three peptides and six receptor subtypes

Article/chapterEnglish2012

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Elsevier BV,2012
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LIBRIS-ID:oai:DiVA.org:uu-129517
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-129517URI
https://doi.org/10.1016/j.ygcen.2012.04.027DOI

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Language:English
Summary in:English

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Erratum in General and Comparative Endocrinology 2015:215, doi:10.1016/j.ygcen.2014.11.014.
Neuropeptide Y and its related peptides PYY and PP (pancreatic polypeptide) are involved in feeding behavior, regulation of the pituitary and the gastrointestinal tract, and numerous other functions. The peptides act on a family of G-protein coupled receptors with 4-7 members in jawed vertebrates. We describe here the NPY system of the Western clawed frog Silurana (Xenopus) tropicalis. Three peptides, NPY, PYY and PP, were identified together with six receptors, namely subtypes Y1, Y2, Y4, Y5, Y7 and Y8. Thus, this frog has all but one of the ancestral seven gnathostome NPY-family receptors, in contrast to mammals which have lost 2-3 of the receptors. Expression levels of mRNA for the peptide and receptor genes were analyzed in a panel of 19 frog tissues using reverse transcriptase quantitative PCR. The peptide mRNAs had broad distribution with highest expression in skin, blood and small intestine. NPY mRNA was present in the three brain regions investigated, but PYY and PP mRNAs were not detectable in any of these. All receptor mRNAs had similar expression profiles with high expression in skin, blood, muscle and heart. Three of the receptors, Y5, Y7 and Y8, could be functionally expressed in HEK-293 cells and characterized with binding studies using the three frog peptides. PYY had the highest affinity for all three receptors (K(i) 0.042-0.34 nM). Also NPY and PP bound to the Y8 receptor with high affinity (0.14 and 0.50 nM). The low affinity of NPY for the Y5 receptor (100-fold lower than PYY) differs from mammals and chicken. This may suggest a less important role of NPY on Y5 in appetite stimulation in the frog compared with amniotes. In conclusion, our characterization of the NPY system in S. tropicalis with its six receptors demonstrates not only greater complexity than in mammals but also some interesting differences in ligand-receptor preferences.

Subject headings and genre

NATURVETENSKAP Biologi hsv//swe
NATURAL SCIENCES Biological Sciences hsv//eng
NPY
PYY
G-protein-coupled receptor
Silurana tropicalis
evolution

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Xu, BoUppsala universitet,Farmakologi,Larhammar(Swepub:uu)boaxu109 (author)
Larsson, Tomas AUppsala universitet,Farmakologi,Larhammar (author)
Heldin, JohanUppsala universitet,Institutionen för neurovetenskap,Larhammar(Swepub:uu)johel535 (author)
Bergqvist, Christina AUppsala universitet,Farmakologi,Larhammar(Swepub:uu)cbe04091 (author)
Fredriksson, RobertUppsala universitet,Farmakologi,Larhammar(Swepub:uu)rfr20930 (author)
Conlon, JMDept of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University (author)
Lundell, IngridUppsala universitet,Farmakologi,Larhammar(Swepub:uu)ilu21838 (author)
Denver, RJDepartment of Molecular, Cellular and Developmental Biology, The University of Michigan, 3065C Kraus Building, Ann Arbor, MI 48109-1048, USA (author)
Larhammar, DanUppsala universitet,Farmakologi,Larhammar(Swepub:uu)dla05000 (author)
Uppsala universitetInstitutionen för neurovetenskap (creator_code:org_t)

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In:General and Comparative Endocrinology: Elsevier BV177:3, s. 322-3310016-64801095-6840

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By the author/editor: Sundström, Görel; Xu, Bo; Larsson, Tomas A; Heldin, Johan; Bergqvist, Chris ...; Fredriksson, Rob ...; show more...; Conlon, JM; Lundell, Ingrid; Denver, RJ; Larhammar, Dan; show less...

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