Target identification of small noncoding RNAs in bacteria

• Small noncoding RNAs have been discovered at a staggering rate in Escherichia coli and many other bacteria. Most of the sRNAs of known function regulate gene expression by binding to specific mRNAs or proteins. Given the scores of sRNAs of unknown function, the identification of their cellular targets has become urgent. Here, we review the diverse strategies that have been used to identify and validate bacterial sRNA targets. These include the pulse-expression of sRNAs followed by global transcriptome analysis (microarrays), new biocomputational prediction algorithms, and novel gfp reporter gene fusions to validate candidate target gene regulation.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Base Sequence

Computational Biology/methods

Models; Genetic

Oligonucleotide Array Sequence Analysis

RNA; Bacterial/analysis/genetics/*metabolism

RNA; Messenger/analysis/genetics/metabolism

RNA; Untranslated/analysis/genetics/*metabolism

Biology

Biologi

Publikations- och innehållstyp

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