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Gene expression ana...
Gene expression analysis by cDNA microarray in oral cancers from two Western populations
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Lunde, Mai Lill Suhr (författare)
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Warnakulasuriya, Saman (författare)
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- Sand, Lars (författare)
- Uppsala universitet,Käkkirurgi
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visa fler...
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- Hirsch, Jan M. (författare)
- Uppsala universitet,Käkkirurgi
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Vasstrand, Endre N. (författare)
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Ibrahim, Salah O. (författare)
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visa färre...
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(creator_code:org_t)
- 2010
- 2010
- Engelska.
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Ingår i: Anticancer Research. - 0250-7005 .- 1791-7530. ; 30:4, s. 1083-1091
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- BACKGROUND: In this work, gene expression profile was examined in 19 cases of oral cancer (OC) obtained from patients from Sweden (n=8) and UK (n=11) and the findings were tested for correlation to patient's clinicopathological data. MATERIALS AND METHODS: Following total RNA extraction, cDNA synthesis, labeling with fluorescent dyes and hybridization to the 21 k human oligonucleotide microarrays, slides were scanned and images were subjected to Genepix and J-Express analysis. Results for selected genes were validated by quantitative reverse transcriptase polymerase chain reaction (Q-RT-PCR). RESULTS: 42 genes were identified as being differentially expressed. These included 39 genes of known functions (such as fatty acid synthase (FASN), 5' nucleotidase, ecto (NT5E), high mobility group AT-hook (HMGA1), and v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS)) and 3 novel genes; 26 (67%) of the 39 genes with known functions were previously reported in oral/head and neck tumors examined from other populations. Hierarchical clustering of the samples using the 42 genes demonstrated that samples mainly clustered in the same population. CONCLUSION: These results illustrate that microarrays can be used to identify distinct patterns of gene expression in different populations, but with no direct association to clinicopathological parameters. The fact that 67% of the 39 genes with known functions found in this work were previously reported in oral/head and neck tumors from other populations provides clear evidence that development of these tumors follows the same biological pathways irrespective of the source of the samples used.
Nyckelord
- Microarrays
- gene expression
- oral cancer
- tobacco
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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