Procoagulant behavior and platelet microparticle generation on nanoporous alumina

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Ferraz, Natalia,1976-Uppsala universitet,Ytbioteknik,Nano-biomaterial research and Tissue Culturing (författare)

Procoagulant behavior and platelet microparticle generation on nanoporous alumina

Artikel/kapitelEngelska2010

Förlag, utgivningsår, omfång ...

2009-07-06
SAGE,2010
printrdacarrier

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LIBRIS-ID:oai:DiVA.org:uu-130496
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-130496URI
https://doi.org/10.1177/0885328209338639DOI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

In the present work, we have investigated platelet microparticle(PMP) generation in whole blood after contact with nanoporous alumina.Alumina membranes with pore sizes of 20 and 200nm in diameter were incubated with whole blood and the number of PMP in the fluid phase was determined by flow cytometry. The role of the complement system in PMP generation was investigated using an analog of the potent complement inhibitor compstatin. Moreover, the procoagulant activity of the two pore size membranes were compared by measuring thrombin formation. Results indicated that PMP were not present in the fluid phase after whole blood contact with either of the alumina membranes. However, scanning electron microscope micrographs clearly showed the presence of PMP clusters on the 200nm pore size alumina, while PMP were practically absent on the 20nm membrane. We probed no influence of complement activation in PMP generation and adhesion and we hypothesize that other specific material-related protein–platelet interactions are taking place. A clear difference in procoagulant activity between the membranes could also be seen, 20nm alumina showed 100% higher procoagulant activity than 200nm membrane. By combining surface evaluation and flow cytometry analyses of the fluid phase, we are able to conclude that 200nm pore size alumina promotes PMP generation and adhesion while the 20nm membrane does not appreciably cause any release or adhesion of PMP, thus indicating a direct connection between PMP generation and nanoporosity.

Ämnesord och genrebeteckningar

NATURVETENSKAP Kemi hsv//swe
NATURAL SCIENCES Chemical Sciences hsv//eng
nanoporous alumina
nanotopography
platelets
platelet microparticles
procoagulant activity
compstatin
Chemistry
Kemi
Immunologi
Immunology
Materialvetenskap
Materials Science

Biuppslag (personer, institutioner, konferenser, titlar ...)

Hong, JaanUppsala universitet,Enheten för klinisk immunologi(Swepub:uu)jaanhong (författare)
Karlsson Ott, Marjam,1975-Uppsala universitet,Ytbioteknik,Nano-biomaterial research and Tissue Culturing(Swepub:uu)makar516 (författare)
Uppsala universitetYtbioteknik (creator_code:org_t)

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Ingår i:Journal of biomaterials applications: SAGE24:8, s. 675-6920885-32821530-8022

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Av författaren/redakt...: Ferraz, Natalia, ...; Hong, Jaan; Karlsson Ott, Ma ...

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NATURVETENSKAP: NATURVETENSKAP; och Kemi

Artiklar i publikationen: Journal of bioma ...

Av lärosätet: Uppsala universitet

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