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Search: L773:1862 8354 OR L773:1862 8346 > (2010-2014) > MS analysis of rheu...

  • Hermansson, Monika (author)

MS analysis of rheumatoid arthritic synovial tissue identifies specific citrullination sites on fibrinogen.

  • Article/chapterEnglish2010

Publisher, publication year, extent ...

  • 2010-01-25
  • Wiley,2010
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-136069
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-136069URI
  • https://doi.org/10.1002/prca.200900088DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:120565229URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Purpose Citrullination is a post-translational modification of arginine residues to citrulline catalyzed by peptidyl arginine deiminases. Induced expression of citrullinated proteins are frequently detected in various inflammatory states including arthritis; however, direct detection of citrullination in arthritic samples has not been successfully performed in the past. Experimental design Citrullination of human fibrinogen, a candidate autoantigen in arthritis, was studied. Accurate identification of citrullinated fibrinogen peptides from rheumatoid arthritis synovial tissue specimens was performed using accurate mass and retention time analysis. Results A peptide with the sequence ESSSHHPGIAEFPSRGK corresponding to amino acids 559-575 of fibrinogen a-chain was identified to be citrullinated with an occupancy rate between 1.4 and 2.5%. Citrullination of the peptide KREEAPSLRPAPPPISGGGYRARPAK corresponding to amino acids 52-77 of the fibrinogen beta-chain was identified with an occupancy rate of 1.2%. Conclusions and clinical relevance We report a proof of principle study for the identification of citrullinated proteins and within them, identification of citrullination sites and quantification of their occupancies in synovial tissue from rheumatoid arthritis patients using high-resolution MS. Detailed studies on which molecules are citrullinated in arthritis can provide information about their role in immune regulation and serve as novel biomarkers and potentially even as therapeutic targets.

Subject headings and genre

  • Citrullination
  • fibrinogen
  • MS
  • rheumatoid arthritis
  • MEDICINE
  • MEDICIN

Added entries (persons, corporate bodies, meetings, titles ...)

  • Artemenko, KonstantinUppsala universitet,Institutionen för cell- och molekylärbiologi(Swepub:uu)konar476 (author)
  • Ossipova, ElenaKarolinska Institutet (author)
  • Eriksson, HannaKarolinska Institutet (author)
  • Lengqvist, JohanKarolinska Institutet (author)
  • Makrygiannakis, Dimitrios (author)
  • Catrina, Anca I.Karolinska Institutet (author)
  • Nicholas, Anthony P. (author)
  • Klareskog, LarsKarolinska Institutet (author)
  • Savitski, MikhailUppsala universitet,Institutionen för cell- och molekylärbiologi (author)
  • Zubarev, Roman A.Karolinska Institutet,Uppsala universitet,Institutionen för cell- och molekylärbiologi(Swepub:uu)rozub839 (author)
  • Jakobsson, Per-JohanKarolinska Institutet (author)
  • Uppsala universitetInstitutionen för cell- och molekylärbiologi (creator_code:org_t)

Related titles

  • In:Proteomics - Clinical Applications: Wiley4:5, s. 511-5181862-83541862-8346

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