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Tissue localization...
Tissue localization and the establishment of a sensitive immunoassay of the newly discovered human phospholipase B-precursor (PLB-P)
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- Xu, Shengyuan (författare)
- Uppsala universitet,Klinisk kemi
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- Cai, Linjun (författare)
- Uppsala universitet,Klinisk kemi
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- Zhao, Linshu (författare)
- Uppsala universitet,Klinisk kemi
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- Douhan-Håkansson, Lena (författare)
- Uppsala universitet,Klinisk kemi
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Kristjansson, Gudjon (författare)
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- Pauksen, Karlis (författare)
- Uppsala universitet,Infektionssjukdomar
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- Venge, Per (författare)
- Uppsala universitet,Klinisk kemi
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(creator_code:org_t)
- Elsevier BV, 2010
- 2010
- Engelska.
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Ingår i: JIM - Journal of Immunological Methods. - : Elsevier BV. - 0022-1759 .- 1872-7905. ; 353:1-2, s. 71-77
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Human phospholipase B-precursor (PLB-P) is a newly identified and purified protein from human neutrophils. The precise function of PLB-P in vivo is not yet known. Its existence in neutrophils and the enzymatic activity against phospholipids imply a role in the defence against invading microorganisms and in the generation of lipid mediators of inflammation. We describe here the generation of specific antibodies against PLB-P, the tissue localizations of PLB-P and the establishment of an accurate, specific, and reproducible radioimmunoassay (RIA). A survey of normal and malignant tissues showed strong immunostaining of PLB-P in neuronal and myeloid cells and in adrenal glands. Elevated levels were found in sera of patients with influenza A infection i.e. > 1 mu g/L and in gut fluids of patients with inflammatory bowel disease i.e. > 20 mu g/L. The levels correlated to markets of neutrophil activation, suggesting a neutrophil origin of PLB-P in these conditions. The antibodies and the assay will be useful in the future basic and clinical investigations of PLB-P.
Nyckelord
- Phospholipase B
- Radioimmunoassay
- Neutrophils
- Infection
- Inflammation
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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