Impact of matrix properties on survival of freeze-dried bacteria

Wessman, PerSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,Department of Mircobiology, SLU, Swedish University of Agricultural Sciences (author)

Background: Disaccharides are in general first choice as formulation compounds when freezedrying microorganisms. Although polysaccharides and other biopolymers are considered too large to stabilize and interact with cell components in the same beneficial way as disaccharides, polymers have been reported to support cell survival. In the present study we compare the efficiency of sucrose, the polymers Ficoll, hydroxyethylcellulose, hydroxypropylmethylcellulose and polyvinylalcohol to support survival of three bacterial strains during freeze-drying. The initial osmotic conditions were adjusted to be similar for all formulations. Formulation characterization was used to interpret the impact that different compound properties had on cell survival. Results: Despite differences in molecular size, both sucrose and the sucrose based polymer Ficoll supported cell survival after freeze-drying equally well. All formulations became amorphous upon dehydration. Scanning electron microscopy and X-ray diffraction data showed that the discerned differences in structure of the dry formulations had little impact on the survival rates. The capability of the polymers to support cell survival correlated with the surface activity of the polymers in a similar way for all investigated bacterial strains. Conclusion: Polymer-based formulations can support cell survival as effectively as disaccharides if formulation properties of importance for maintaining cell viability are identified and controlled.

LANTBRUKSVETENSKAPER Lantbruksvetenskap, skogsbruk och fiske Livsmedelsvetenskap hsv//swe
AGRICULTURAL SCIENCES Agriculture, Forestry and Fisheries Food Science hsv//eng
formulation
freeze-drying
biopolymer
sucrose
bacteria
surface
Food science
Livsmedelsvetenskap
TECHNOLOGY
TEKNIKVETENSKAP
Biologi med inriktning mot mikrobiologi
Biology with specialization in Microbiology
Teknisk fysik med inriktning mot materialvetenskap
Engineering Science with specialization in Materials Science

Mahlin, DennyUppsala universitet,Institutionen för farmaci(Swepub:uu)dma10394 (author)
Akhtar, SultanUppsala universitet,Tillämpad materialvetenskap(Swepub:uu)sulak695 (author)
Rubino, Stefano,1978-Uppsala universitet,Tillämpad materialvetenskap(Swepub:uu)steru726 (author)
Leifer, Klaus,1965-Uppsala universitet,Tillämpad materialvetenskap(Swepub:uu)kllei839 (author)
Kessler, VadimSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kemi,Department of Chemistry,Department of Chemistry, SLU, Swedish University of Agricultural Sciences(Swepub:slu)46900 (author)
Håkansson, SebastianSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,Department of Microbiology, SLU, Swedish University of Agricultural Sciences(Swepub:slu)48672 (author)
Sveriges lantbruksuniversitetInstitutionen för mikrobiologi (creator_code:org_t)
Sveriges lantbruksuniversitet

In:Journal of the Science of Food and Agriculture: Wiley91:14, s. 2518-25280022-51421097-0010

By the author/editor: Wessman, Per; Mahlin, Denny; Akhtar, Sultan; Rubino, Stefano, ...; Leifer, Klaus, 1 ...; Kessler, Vadim; show more...; Håkansson, Sebas ...; show less...

About the subject

AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Agriculture Fore ...; and Food Science

By the university: Uppsala University; Swedish University of Agricultural Sciences

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