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Peptidoglycan activ...
Peptidoglycan activation of the proPO-system without a peptidoglycan receptor protein (PGRP)?
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- Liu, Haipeng (författare)
- Uppsala universitet,Jämförande fysiologi
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- Wu, Chenglin (författare)
- Uppsala universitet,Jämförande fysiologi
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Matsuda, Yasuyuki (författare)
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Kawabata, Shun-ichiro (författare)
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Lee, Bok Luel (författare)
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- Söderhäll, Kenneth (författare)
- Uppsala universitet,Jämförande fysiologi
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- Söderhäll, Irene (författare)
- Uppsala universitet,Jämförande fysiologi
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(creator_code:org_t)
- Elsevier BV, 2011
- 2011
- Engelska.
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Ingår i: Developmental and Comparative Immunology. - : Elsevier BV. - 0145-305X .- 1879-0089. ; 35:1, s. 51-61
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Recognition of microbial polysaccharide by pattern recognition receptors triggers the prophenoloxidase (proPO) cascade, resulting in melanin synthesis and its deposition on the surface of invading pathogens. Several masquerade-like proteins and serine proteinase homologues have been shown to be involved in the proPO activation in insects. In this study, a novel serine proteinase homologue, Pl-SPH2, was found and isolated as a 30 kDa protein from hemocytes of the freshwater crayfish, Pacifastocus leniusculus, by its binding property to a partially lysozyme digested or TCA-treated insoluble Lysine (Lys)-type pepticloglycan (PGN) and soluble polymeric Lys-type PGN. Two other proteins, the Pl-SPH1 and lipopolysaccharide- and beta-1,3-glucan-bincling protein (LGBP) were also found in the several different PGN-binding assays. However no PGRP homologue was detected. Neither was any putative PGRP found after searching available crustacean sequence databases. If RNA interference of Pl-SPH2, Pl-SPH1 or LGBP in the crayfish hematopoietic tissue cell culture was performed, it resulted in lower PO activity following activation of the proPO-system by soluble Lys-type PGN. Taken together, we report for the first time that Lys-type PGN is a trigger of proPO-system activation in a crustacean and that two Pl-SPlis are involved in this activation possibly by forming a complex with LGBP and without a PGRP.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Nyckelord
- Innate immunity
- Lys-type PGN
- PGRP
- proPO
- SPHs
- Immunology
- Immunologi
- Biology with specialization in Comparative Physiology
- Biologi med inriktning mot jämförande fysiologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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