Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

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Adewumi, Oluseun (författare)

Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Artikel/kapitelEngelska2007

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2007-06-17
Springer Science and Business Media LLC,2007
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LIBRIS-ID:oai:DiVA.org:uu-144062
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-144062URI
https://doi.org/10.1038/nbt1318DOI
http://kipublications.ki.se/Default.aspx?queryparsed=id:115746012URI
https://lup.lub.lu.se/record/691452URI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

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The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
MEDICINE
MEDICIN

Biuppslag (personer, institutioner, konferenser, titlar ...)

Aflatoonian, Behrouz (författare)
Ahrlund-Richter, LarsKarolinska Institutet (författare)
Amit, Michal (författare)
Andrews, Peter W. (författare)
Beighton, Gemma (författare)
Bello, Paul A. (författare)
Benvenisty, Nissim (författare)
Berry, Lorraine S. (författare)
Bevan, Simon (författare)
Blum, Barak (författare)
Brooking, Justin (författare)
Chen, Kevin G. (författare)
Choo, Andre B. H. (author)
Churchill, Gary A. (author)
Corbel, Marie (author)
Damjanov, Ivan (author)
Draper, Jon S. (author)
Dvorak, Petr (author)
Emanuelsson, Katarina (author)
Fleck, Roland A. (author)
Ford, Angela (author)
Gertow, Karin (author)
Gertsenstein, Marina (author)
Gokhale, Paul J. (author)
Hamilton, Rebecca S. (author)
Hampl, Ales (author)
Healy, Lyn E. (author)
Hovatta, OutiKarolinska Institutet (author)
Hyllner, Johan (author)
Imreh, Marta P.Karolinska Institutet,Uppsala universitet,Zoologisk utvecklingsbiologi (author)
Itskovitz-Eldor, Joseph (author)
Jackson, Jamie (author)
Johnson, Jacqueline L. (author)
Jones, Mark (author)
Kee, Kehkooi (author)
King, Benjamin L. (author)
Knowles, Barbara B. (author)
Lako, Majlinda (author)
Lebrin, Franck (author)
Mallon, Barbara S. (author)
Manning, Daisy (author)
Mayshar, Yoav (author)
Mckay, Ronald D. G. (author)
Michalska, Anna E. (author)
Mikkola, Milla (author)
Mileikovsky, Masha (author)
Minger, Stephen L. (author)
Moore, Harry D. (author)
Mummery, Christine L. (author)
Nagy, Andras (author)
Nakatsuji, Norio (author)
O'Brien, Carmel M. (author)
Oh, Steve K. W. (author)
Olsson, Cia (author)
Otonkoski, Timo (author)
Park, Kye-Yoon (author)
Passier, Robert (author)
Patel, Hema (author)
Patel, Minal (author)
Pedersen, Roger (author)
Pera, Martin F. (author)
Piekarczyk, Marian S. (author)
Pera, Renee A. Reijo (author)
Reubinoff, Benjamin E. (author)
Robins, Allan J. (author)
Rossant, Janet (author)
Rugg-Gunn, Peter (author)
Schulz, Thomas C. (author)
Semb, HenrikLund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)endo-hse (author)
Sherrer, Eric S. (author)
Siemen, Henrike (author)
Stacey, Glyn N. (author)
Stojkovic, Miodrag (author)
Suemori, Hirofumi (author)
Szatkiewicz, Jin (author)
Turetsky, Tikva (author)
Tuuri, Timo (author)
van den Brink, Steineke (author)
Vintersten, Kristina (author)
Vuoristo, Sanna (author)
Ward, Dorien (author)
Weaver, Thomas A. (author)
Young, Lesley A. (author)
Zhang, Weidong (author)
Karolinska InstitutetZoologisk utvecklingsbiologi (creator_code:org_t)

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In:Nature Biotechnology: Springer Science and Business Media LLC25:7, s. 803-8161087-01561546-1696

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By the author/editor: Adewumi, Oluseun; Aflatoonian, Beh ...; Ahrlund-Richter, ...; Amit, Michal; Andrews, Peter W ...; Beighton, Gemma; show more...; Bello, Paul A.; Benvenisty, Niss ...; Berry, Lorraine ...; Bevan, Simon; Blum, Barak; Brooking, Justin; Chen, Kevin G.; Choo, Andre B. H ...; Churchill, Gary ...; Corbel, Marie; Damjanov, Ivan; Draper, Jon S.; Dvorak, Petr; Emanuelsson, Kat ...; Fleck, Roland A.; Ford, Angela; Gertow, Karin; Gertsenstein, Ma ...; Gokhale, Paul J.; Hamilton, Rebecc ...; Hampl, Ales; Healy, Lyn E.; Hovatta, Outi; Hyllner, Johan; Imreh, Marta P.; Itskovitz-Eldor, ...; Jackson, Jamie; Johnson, Jacquel ...; Jones, Mark; Kee, Kehkooi; King, Benjamin L ...; Knowles, Barbara ...; Lako, Majlinda; Lebrin, Franck; Mallon, Barbara ...; Manning, Daisy; Mayshar, Yoav; Mckay, Ronald D. ...; Michalska, Anna ...; Mikkola, Milla; Mileikovsky, Mas ...; Minger, Stephen ...; Moore, Harry D.; Mummery, Christi ...; Nagy, Andras; Nakatsuji, Norio; O'Brien, Carmel ...; Oh, Steve K. W.; Olsson, Cia; Otonkoski, Timo; Park, Kye-Yoon; Passier, Robert; Patel, Hema; Patel, Minal; Pedersen, Roger; Pera, Martin F.; Piekarczyk, Mari ...; Pera, Renee A. R ...; Reubinoff, Benja ...; Robins, Allan J.; Rossant, Janet; Rugg-Gunn, Peter; Schulz, Thomas C ...; Semb, Henrik; Sherrer, Eric S.; Siemen, Henrike; Stacey, Glyn N.; Stojkovic, Miodr ...; Suemori, Hirofum ...; Szatkiewicz, Jin; Turetsky, Tikva; Tuuri, Timo; van den Brink, S ...; Vintersten, Kris ...; Vuoristo, Sanna; Ward, Dorien; Weaver, Thomas A ...; Young, Lesley A.; Zhang, Weidong; show less...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; and Medicinska och f ...; and Cell och molekyl ...

Articles in the publication: Nature Biotechno ...

By the university: Uppsala University; Karolinska Institutet; Lund University

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