Single mimivirus particles intercepted and imaged with an X-ray laser

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MARC

Seibert, M. MarvinUppsala universitet,Molekylär biofysik (author)

Single mimivirus particles intercepted and imaged with an X-ray laser

Article/chapterEnglish2011

Publisher, publication year, extent ...

2011-02-02
Springer Science and Business Media LLC,2011
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:uu-146069
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-146069URI
https://doi.org/10.1038/nature09748DOI
https://res.slu.se/id/publ/33573URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions(1-4). Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma(1). The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval(2). Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a noncrystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source(5). Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000 K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.

Subject headings and genre

NATURVETENSKAP Biologi hsv//swe
NATURAL SCIENCES Biological Sciences hsv//eng
NATURVETENSKAP Biologi Biofysik hsv//swe
NATURAL SCIENCES Biological Sciences Biophysics hsv//eng
NATURVETENSKAP Fysik Atom- och molekylfysik och optik hsv//swe
NATURAL SCIENCES Physical Sciences Atom and Molecular Physics and Optics hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Biology
Biologi

Added entries (persons, corporate bodies, meetings, titles ...)

Ekeberg, TomasUppsala universitet,Molekylär biofysik(Swepub:uu)tomek611 (author)
Maia, Filipe R. N. C.Uppsala universitet,Molekylär biofysik(Swepub:uu)cajca839 (author)
Svenda, MartinUppsala universitet,Molekylär biofysik(Swepub:uu)msv31659 (author)
Andreasson, JakobUppsala universitet,Molekylär biofysik(Swepub:uu)jakan998 (author)
Joensson, OlofUppsala universitet,Molekylär biofysik (author)
Odic, DuskoUppsala universitet,Molekylär biofysik (author)
Iwan, BiancaUppsala universitet,Molekylär biofysik(Swepub:uu)biaiw419 (author)
Rocker, AndreaUppsala universitet,Molekylär biofysik (author)
Westphal, DanielUppsala universitet,Molekylär biofysik(Swepub:uu)danwe345 (author)
Hantke, MaxUppsala universitet,Molekylär biofysik(Swepub:uu)maxha207 (author)
DePonte, Daniel P. (author)
Barty, Anton (author)
Schulz, Joachim (author)
Gumprecht, Lars (author)
Coppola, Nicola (author)
Aquila, Andrew (author)
Liang, Mengning (author)
White, Thomas A. (author)
Martin, Andrew (author)
Caleman, CarlUppsala universitet,Molekylär biofysik(Swepub:uu)cajca839 (author)
Stern, Stephan (author)
Abergel, Chantal (author)
Seltzer, Virginie (author)
Claverie, Jean-Michel (author)
Bostedt, Christoph (author)
Bozek, John D. (author)
Boutet, Sebastien (author)
Miahnahri, A. Alan (author)
Messerschmidt, Marc (author)
Krzywinski, Jacek (author)
Williams, Garth (author)
Hodgson, Keith O. (author)
Bogan, Michael J. (author)
Hampton, Christina Y. (author)
Sierra, Raymond G. (author)
Starodub, Dmitri (author)
Andersson, IngerSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för molekylärbiologi,Department of Molecular Biology(Swepub:slu)47211 (author)
Bajt, Sasa (author)
Barthelmess, Miriam (author)
Spence, John C. H. (author)
Fromme, Petra (author)
Weierstall, Uwe (author)
Kirian, Richard (author)
Hunter, Mark (author)
Doak, R. Bruce (author)
Marchesini, Stefano (author)
Hau-Riege, Stefan P. (author)
Frank, Matthias (author)
Shoeman, Robert L. (author)
Lomb, Lukas (author)
Epp, Sascha W. (author)
Hartmann, Robert (author)
Rolles, Daniel (author)
Rudenko, Artem (author)
Schmidt, Carlo (author)
Foucar, Lutz (author)
Kimmel, Nils (author)
Holl, Peter (author)
Rudek, Benedikt (author)
Erk, Benjamin (author)
Hoemke, Andre (author)
Reich, Christian (author)
Pietschner, Daniel (author)
Weidenspointner, Georg (author)
Strueder, Lothar (author)
Hauser, Guenter (author)
Gorke, Hubert (author)
Ullrich, Joachim (author)
Schlichting, Ilme (author)
Herrmann, Sven (author)
Schaller, Gerhard (author)
Schopper, Florian (author)
Soltau, Heike (author)
Kuehnel, Kai-Uwe (author)
Andritschke, Robert (author)
Schroeter, Claus-Dieter (author)
Krasniqi, Faton (author)
Bott, Mario (author)
Schorb, Sebastian (author)
Rupp, Daniela (author)
Adolph, Marcus (author)
Gorkhover, Tais (author)
Hirsemann, Helmut (author)
Potdevin, Guillaume (author)
Graafsma, Heinz (author)
Nilsson, Björn (author)
Chapman, Henry N. (author)
Hajdu, JanosUppsala universitet,Molekylär biofysik(Swepub:uu)janohajd (author)
Uppsala universitetMolekylär biofysik (creator_code:org_t)
Sveriges lantbruksuniversitet

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In:Nature: Springer Science and Business Media LLC470:7332, s. 78-810028-08361476-4687

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By the author/editor: Seibert, M. Marv ...; Ekeberg, Tomas; Maia, Filipe R. ...; Svenda, Martin; Andreasson, Jako ...; Joensson, Olof; show more...; Odic, Dusko; Iwan, Bianca; Rocker, Andrea; Westphal, Daniel; Hantke, Max; DePonte, Daniel ...; Barty, Anton; Schulz, Joachim; Gumprecht, Lars; Coppola, Nicola; Aquila, Andrew; Liang, Mengning; White, Thomas A.; Martin, Andrew; Caleman, Carl; Stern, Stephan; Abergel, Chantal; Seltzer, Virgini ...; Claverie, Jean-M ...; Bostedt, Christo ...; Bozek, John D.; Boutet, Sebastie ...; Miahnahri, A. Al ...; Messerschmidt, M ...; Krzywinski, Jace ...; Williams, Garth; Hodgson, Keith O ...; Bogan, Michael J ...; Hampton, Christi ...; Sierra, Raymond ...; Starodub, Dmitri; Andersson, Inger; Bajt, Sasa; Barthelmess, Mir ...; Spence, John C. ...; Fromme, Petra; Weierstall, Uwe; Kirian, Richard; Hunter, Mark; Doak, R. Bruce; Marchesini, Stef ...; Hau-Riege, Stefa ...; Frank, Matthias; Shoeman, Robert ...; Lomb, Lukas; Epp, Sascha W.; Hartmann, Robert; Rolles, Daniel; Rudenko, Artem; Schmidt, Carlo; Foucar, Lutz; Kimmel, Nils; Holl, Peter; Rudek, Benedikt; Erk, Benjamin; Hoemke, Andre; Reich, Christian; Pietschner, Dani ...; Weidenspointner, ...; Strueder, Lothar; Hauser, Guenter; Gorke, Hubert; Ullrich, Joachim; Schlichting, Ilm ...; Herrmann, Sven; Schaller, Gerhar ...; Schopper, Floria ...; Soltau, Heike; Kuehnel, Kai-Uwe; Andritschke, Rob ...; Schroeter, Claus ...; Krasniqi, Faton; Bott, Mario; Schorb, Sebastia ...; Rupp, Daniela; Adolph, Marcus; Gorkhover, Tais; Hirsemann, Helmu ...; Potdevin, Guilla ...; Graafsma, Heinz; Nilsson, Björn; Chapman, Henry N ...; Hajdu, Janos; show less...

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...; and Atom and Molecul ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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