Effects of Congo Red on A beta(1-40) Fibril Formation Process and Morphology

• Alzheimer's disease (AD), an age-related neurodegenerative disorder, is the most common form of dementia, and the seventh-leading cause of death in the United States. Current treatments offer only symptomatic relief; thus, there is a great need for new treatments with disease-modifying potential. One pathological hallmark of AD is so-called senile plaques, mainly made up of beta-sheet-rich assemblies of 40- or 42-residue amyloid beta-peptides (A beta). Hence, inhibition of A beta aggregation is actively explored as an option to prevent or treat AD. Congo red (CR) has been widely used as a model antiamyloid agent to prevent A beta aggregation. Herein, we report detailed morphological studies on the effect of CR as an antiamyloid agent, by circular dichroism spectroscopy, photo-induced cross-linking reactions, and atomic force microscopy. We also demonstrate the effect of CR on a preaggregated sample of A beta(1-40). Our result suggests that A beta(1-40) follows a different path for aggregation in the presence of CR.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Alzheimer's disease

atomic force microscopy

Congo red

amyloid

oligomers

fibrils

aggregates

amyloid beta-peptide

Chemistry

Kemi

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)