Search: WFRF:(Sandström A.) >
The NMR structure o...
The NMR structure of 31-mer RNA domain of E. coli RNase P RNA using its non-uniformly deuterium labelled counterpart (the "NMR-window" concept)
- Article/chapterEnglish1996
Publisher, publication year, extent ...
-
Oxford University Press (OUP),1996
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-147823
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-147823URI
-
https://doi.org/10.1093/nar/24.11.2022DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
The NMR structure of a 31mer RNA constituting a functionally important domain of the catalytic RNase P RNA from Escherichia coli is reported. Severe spectral overlaps of the proton resonances in the natural 31mer RNA (1) were successfully tackled by unique spectral simplifications found in the partially-deuterated 31 mer RNA analogue (2) incorporating deuterated cytidines [C5 (>95 atom % 2H), C2' (>97 atom % 2H), C3' (>97 atom % 2H), C4' (>65 atom % 2H) and C5' (>97 atom % 2H)] [for the 'NMR-window' concept see: Földesi,A. et al. (1992) Tetrahedron, 48, 9033; Foldesi,A. et al. (1993) J. Biochem. Biophys. Methods, 26, 1; Yamakage,S.-I. et al. (1993) Nucleic Acids Res., 21, 5005; Agback,P. et al. (1994) Nucleic Acids Res., 22, 1404; Földesi,A. et al. (1995) Tetrahedron, 51, 10065; Földesi,A. et al. (1996) Nucleic Acids Res., 24, 1187-1194]. 175 resonances have been assigned out of total of 235 non-exchangeable proton resonances in (1) in an unprecedented manner in the absence of 13C and 15N labelling. 41 out of 175 assigned resonances could be accomplished with the help of the deuterated analogue (2). The two stems in 31mer RNA adopt an A-type RNA conformation and the base-stacking continues from stem I into the beginning of the loop I. Long distance cross-strand NOEs showed a structured conformation at the junction between stem I and loop I. The loop I-stem II junction is less ordered and shows structural perturbation at and around the G11 -C22 base pair.
Subject headings and genre
-
NATURAL SCIENCES
-
NATURVETENSKAP
Added entries (persons, corporate bodies, meetings, titles ...)
-
Kufel, J
(author)
-
Földesi, A
(author)
-
Maltseva, T
(author)
-
Sandström, A
(author)
-
Kirsebom, Leif AUppsala universitet,Mikrobiologi
(author)
-
Chattopadhyaya, J
(author)
-
Uppsala universitetMikrobiologi
(creator_code:org_t)
Related titles
-
In:Nucleic Acids Research: Oxford University Press (OUP)24:11, s. 2022-20350305-10481362-4962
Internet link
Find in a library
To the university's database