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Impact of Temperatu...
Impact of Temperature Dependent Sampling Procedures in Proteomics and Peptidomics : A Characterization of the Liver and Pancreas Post Mortem Degradome
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- Scholz, Birger (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Division of Toxicology
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Sköld, Karl (author)
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- Kultima, Kim (author)
- Uppsala universitet,Klinisk farmakologi
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- Fernandez, Celine (author)
- Lund University,Lunds universitet,Kardiovaskulär forskning - hypertoni,Forskargrupper vid Lunds universitet,Cardiovascular Research - Hypertension,Lund University Research Groups
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- Waldemarson, Sofia (author)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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- Savitski, Mikhail M. (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Molecular Biometry Group
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Söderquist, Marcus (author)
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Borén, Mats (author)
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Stella, Robert (author)
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Andrén, Per (author)
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- Zubarev, Roman (author)
- Karolinska Institutet,Uppsala universitet,Institutionen för cell- och molekylärbiologi,Molecular Biometry Group
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- James, Peter (author)
- Lund University,Lunds universitet,Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Division of Toxicology,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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Svensson, Marcus (author)
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(creator_code:org_t)
- 2011
- 2011
- English.
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In: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 10:3, s. M900229MCP200-
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Little is known about the nature of post mortem degradation of proteins and peptides on a global level, the so-called degradome. This is especially true for nonneural tissues. Degradome properties in relation to sampling procedures on different tissues are of great importance for the studies of, for instance, post translational modifications and/or the establishment of clinical biobanks. Here, snap freezing of fresh(< 2 min post mortem time) mouse liver and pancreas tissue is compared with rapid heat stabilization with regard to effects on the proteome (using two-dimensional differential in-gel electrophoresis) and peptidome (using label free liquid chromatography). We report several proteins and peptides that exhibit heightened degradation sensitivity, for instance superoxide dismutase in liver, and peptidyl-prolyl cis-trans isomerase and insulin C-peptides in pancreas. Tissue sampling based on snap freezing produces a greater amount of degradation products and lower levels of endogenous peptides than rapid heat stabilization. We also demonstrate that solely snap freezing related degradation can be attenuated by subsequent heat stabilization. We conclude that tissue sampling involving a rapid heat stabilization step is preferable to freezing with regard to proteomic and peptidomic sample quality.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Scholz, Birger
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Sköld, Karl
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Kultima, Kim
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Fernandez, Celin ...
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Waldemarson, Sof ...
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Savitski, Mikhai ...
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show more...
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Söderquist, Marc ...
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Borén, Mats
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Stella, Robert
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Andrén, Per
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Zubarev, Roman
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James, Peter
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Svensson, Marcus
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Molecular & Cell ...
- By the university
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Uppsala University
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Karolinska Institutet
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Lund University